猪血清铁蛋白的固相免疫放射分析

Solid phase immunoradiometric assay for porcine serum ferritin.

Adams P C, Powell L W, Halliday J W

Department of Medicine, University of Queensland, Royal Brisbane Hospital, Brisbane, Australia.

Comp Biochem Physiol B. 1988;89(2):355-8. doi: 10.1016/0305-0491(88)90236-2.

Abstract

A solid phase immunoradiometric assay using anti-serum coated polystyrene tubes, is described for the assay of porcine serum ferritin. 2. The mean concentration of ferritin in the serum of both male and female pigs (Sus scrofa) was 12.1 micrograms/l +/- 8.7 micrograms (range less than 1-35 micrograms/l) and no sex differences were observed in 40 pigs from 1 day to 4 years old. 3. Serum ferritin increased with increasing body iron stores in iron loaded pigs as assessed by hepatic iron concentration. 4. The assay is sensitive (detecting less than 1 microgram/l), reproducible, specific and it does not cross-react with human or rat ferritin.

摘要

本文描述了一种使用抗血清包被聚苯乙烯管的固相免疫放射分析方法，用于测定猪血清铁蛋白。2. 雄性和雌性猪（野猪）血清中铁蛋白的平均浓度为12.1微克/升±8.7微克（范围小于1 - 35微克/升），在40头1日龄至4岁的猪中未观察到性别差异。3. 通过肝脏铁浓度评估，铁负荷猪的血清铁蛋白随着体内铁储存量的增加而增加。4. 该分析方法灵敏（可检测到小于1微克/升）、可重复、特异，且不与人或大鼠铁蛋白发生交叉反应。