Halliday J W, Gera K L, Powell L W
Clin Chim Acta. 1975 Feb 8;58(3):207-14. doi: 10.1016/0009-8981(75)90439-8.
A solid-phase "direct" radio immunoassay which is highly sensitive (detecting less than 0.5 ng/ml) and reproducible is described for the assay of ferritin in human serum. The method makes use of specific anti-ferritin antibody prepared using human liver ferritin; It has the advantage that tubes coated with specific antiserum may be stored for later use and lends itself readily to an automated washing procedure. The concentration of ferritin present in the serum of normal males in the population was 56,9 ng/ml (geometric mean) with a 95 percent confidence range of 18 to 180 ng/ml. In normal females the concentration was 34.0 ng/ml (geometric mean) with a 95 percent condidence range of 10 to 143 ng/ml.
本文描述了一种用于检测人血清中铁蛋白的固相“直接”放射免疫测定法，该方法高度灵敏（可检测到低于0.5纳克/毫升的铁蛋白）且具有可重复性。该方法利用用人肝铁蛋白制备的特异性抗铁蛋白抗体；其优点是涂有特异性抗血清的试管可储存以备后用，并且易于采用自动洗涤程序。该人群中正常男性血清中铁蛋白的浓度为56.9纳克/毫升（几何平均值），95%置信区间为18至180纳克/毫升。正常女性的浓度为34.0纳克/毫升（几何平均值），95%置信区间为10至143纳克/毫升。
