在无细胞系统中，受体介导的内吞作用途径来源的囊泡之间的融合。
Fusion between vesicles from the pathway of receptor-mediated endocytosis in a cell-free system.
作者信息
Woodman P G, Warren G
机构信息
Department of Biochemistry, University of Dundee, Scotland.
出版信息
Eur J Biochem. 1988 Apr 5;173(1):101-8. doi: 10.1111/j.1432-1033.1988.tb13972.x.
Abstract
Fusion between endocytic vesicles containing 125I-transferrin and ones containing anti-transferrin antibody was monitored by the formation of an immune complex. Fusion required ATP and only occurred between specific populations of vesicles. Cytosol was also required. Trypsinisation of the preparations or pre-treatment with N-ethylmaleimide abolished the fusion reaction.
摘要
通过免疫复合物的形成来监测含有¹²⁵I-转铁蛋白的内吞小泡与含有抗转铁蛋白抗体的内吞小泡之间的融合。融合需要ATP，并且只发生在特定的小泡群体之间。还需要胞质溶胶。对制剂进行胰蛋白酶处理或用N-乙基马来酰亚胺预处理会消除融合反应。
Zotero 插件