Water, Biodiversity and Climate change Laboratory, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Av. Prince My Abdellah, P.O. Box 2390, Marrakech 40000, Morocco.
Bioactives, Health and Environement Laboratory, "Biology, Environement & Health" Research Unit, Department of Biology, Faculty of Sciences and technology, Moulay Ismail University, B.P. 509 Boutalamine, Errachidia 52000, Morocco.
Toxins (Basel). 2021 Feb 5;13(2):118. doi: 10.3390/toxins13020118.
Microcystins (MCs) produced in eutrophic waters may decrease crop yield, enter food chains and threaten human and animal health. The main objective of this research was to highlight the role of rhizospheric soil microbiota to protect faba bean plants from MCs toxicity after chronic exposure. Faba bean seedlings were grown in pots containing agricultural soil, during 1 month under natural environmental conditions of Marrakech city in Morocco (March-April 2018) and exposed to cyanobacterial extracts containing up to 2.5 mg·L of total MCs. Three independent exposure experiments were performed (a) agricultural soil was maintained intact "exposure experiment 1"; (b) agricultural soil was sterilized "exposure experiment 2"; (c) agricultural soil was sterilized and inoculated with the rhizobia strain RhOF34 "exposure experiment 3". Overall, data showed evidence of an increased sensitivity of faba bean plants, grown in sterilized soil, to MCs in comparison to those grown in intact and inoculated soils. The study revealed the growth inhibition of plant shoots in both exposure experiments 2 and 3 when treated with 2.5 mg·L of MCs. The results also showed that the estimated daily intake (EDI) of MCs, in sterilized soil, exceeded 2.18 and 1.16 times the reference concentrations (0.04 and 0.45 µg of microcysin-leucine arginine (MC-LR). Kg DW) established for humans and cattle respectively, which raises concerns about human food chain contamination.
微囊藻毒素（MCs）在富营养化水中产生，可能会降低作物产量，进入食物链，并威胁人类和动物健康。本研究的主要目的是强调根际土壤微生物群落的作用，以保护蚕豆植物免受 MCs 毒性的慢性暴露。蚕豆幼苗在含有农业土壤的盆中生长，在摩洛哥马拉喀什市的自然环境条件下（2018 年 3 月至 4 月）进行了为期 1 个月的生长，并暴露于含有高达 2.5mg·L 的总 MCs 的蓝藻提取物中。进行了三个独立的暴露实验（a）保持原状的农业土壤“暴露实验 1”；（b）农业土壤消毒“暴露实验 2”；（c）农业土壤消毒并用根瘤菌菌株 RhOF34 接种“暴露实验 3”。总体而言，数据表明，与在完整和接种土壤中生长的蚕豆植物相比，在消毒土壤中生长的蚕豆植物对 MCs 的敏感性增加。研究表明，当用 2.5mg·L 的 MCs 处理时，暴露实验 2 和 3 中的植物地上部分的生长均受到抑制。结果还表明，在消毒土壤中，MCs 的估计每日摄入量（EDI）超过了人类和牛分别建立的参考浓度（0.04 和 0.45µg 微囊藻素-亮氨酸-精氨酸（MC-LR）。公斤 DW）的 2.18 和 1.16 倍，这引起了对人类食物链污染的关注。
