Lyon M F, Zenthon J, Evans E P, Burtenshaw M D, Willison K R
M.R.C. Radiobiology Unit, Chilton, Didcot, Oxon, UK.
Immunogenetics. 1988;27(5):375-82. doi: 10.1007/BF00395134.
Probes for loci situated near one end of the proximal (Tcp-1) and distal (Qa-2, 3) inversions of the mouse t complex have been hybridized to chromosomes of mice with and without t complexes and with morphologically distinguishable chromosome 17s. Both the probe for Tcp-1 and that for Qa-2, 3 hybridized to clearly different positions on t and non-t chromosomes, thus making visible the extent of the two inversions. The proximal inversion extends from roughly the junction of bands A1 and A2 to band A3, and the distal inversion from band A3 to band C. Thus, the whole t complex extends from the band A1-A2 junction to band C, and is therefore somewhat larger than previously thought, and occupies about 1.2% of the genome. A probe for complement component 3 (C3-1), genetically known to be several cM distal to the t complex, was found by in situ hybridization to lie in band E1. The proximal part of chromosome 17 is one of the best known parts of the mouse genome, at both the genetic and molecular levels. It may soon be possible to correlate the length of the t complex in terms of chromosomal distance with its physical length in megabases.
针对位于小鼠t复合体位点近端（Tcp-1）和远端（Qa-2、3）倒位一端附近基因座的探针，已与带有和不带有t复合体以及具有形态学可区分的17号染色体的小鼠染色体进行杂交。Tcp-1探针和Qa-2、3探针均与t染色体和非t染色体上明显不同的位置杂交，从而使两个倒位的范围可见。近端倒位从大致A1和A2带的交界处延伸到A3带，远端倒位从A3带延伸到C带。因此，整个t复合体从A1-A2带交界处延伸到C带，因此比之前认为的要大一些，约占基因组的1.2%。已知在遗传学上位于t复合体远端几个厘摩处的补体成分3（C3-1）探针，通过原位杂交发现位于E1带。17号染色体的近端部分在遗传和分子水平上都是小鼠基因组中最知名的部分之一。很快就有可能将t复合体在染色体距离方面的长度与其以兆碱基为单位的物理长度关联起来。
