Soleymanifard Sh, Rostamyari M, B Rassouli F, Mehdizadeh A R
PhD, Medical Physics Research Center, Mashhad University of Medical Science, Mashhad, Iran.
MSc, Department of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran.
J Biomed Phys Eng. 2021 Feb 1;11(1):1-8. doi: 10.31661/jbpe.v0i0.2001-1051. eCollection 2021 Feb.
Radiotherapy has become a part of therapeutic process of more than 50 percent of patients suffering from cancer. However, recent studies have shown that radiation therapy might affect the expression of adhesive molecule related genes such as E-cadherin and cause cancer cells to move and migrate. Besides, various studies have reported that the expression of E-cadherin changes differently after radiation treatment. There are several studies which showed the loss of E-cadherin function after radiation; however, this reduction has not been observed in others.
This study aims to investigate the effect of different radiation doses of X-ray on changes that might occur in the expression of E-cadherin gene in colorectal cancer cell line HT-29.
In this experimental study, the cells cultured in flasks were irradiated with X- rays in different doses, including 0.1, 2.5, 5, and 10 Gy; then, the expression of E-cadherin gene was measured using real-time PCR.
The expression of E-cadherin did not change significantly in post-irradiated HT-29 cell line after different radiation doses of X-ray.
The results showed that low, medium and high doses of X- radiation did not change the expression of E-cadherin gene in HT-29 cancer cells. However, it has been reported that radiation mostly downregulated the expression of E-cadherin and mediated metastasis formation and invasiveness in different cancer cell lines. Therefore, further studies need to be conducted to investigate the effects of radiation dose on the molecular pathways contributing to regulation of E-cadherin in HT-29 cell line.
放射治疗已成为超过50%癌症患者治疗过程的一部分。然而，最近的研究表明，放射治疗可能会影响黏附分子相关基因（如E-钙黏蛋白）的表达，并导致癌细胞移动和迁移。此外，各种研究报告称，放射治疗后E-钙黏蛋白的表达变化各不相同。有几项研究表明放射后E-钙黏蛋白功能丧失；然而，在其他研究中并未观察到这种降低。
本研究旨在探讨不同剂量X射线对结肠癌细胞系HT-29中E-钙黏蛋白基因表达可能发生的变化的影响。
在本实验研究中，用不同剂量（包括0.1、2.5、5和10 Gy）的X射线照射培养在培养瓶中的细胞；然后，使用实时PCR测量E-钙黏蛋白基因的表达。
在不同剂量X射线照射后的HT-29细胞系中，E-钙黏蛋白的表达没有显著变化。
结果表明，低、中、高剂量的X射线辐射并未改变HT-29癌细胞中E-钙黏蛋白基因的表达。然而，据报道，辐射大多下调E-钙黏蛋白的表达，并介导不同癌细胞系中的转移形成和侵袭性。因此，需要进一步研究来探讨辐射剂量对HT-29细胞系中E-钙黏蛋白调控分子途径的影响。
