Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Department of Stomatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510515, China.
Biomed Res Int. 2021 Jan 29;2021:6658498. doi: 10.1155/2021/6658498. eCollection 2021.
The aim of this study is to evaluate the biological safety of tantalum (Ta) particles and to further explore the effects of Ta particles on human monocyte toxicity and inflammatory cytokine expression. Human monocyte leukemia (THP-1) cells were cultured with Ta and hydroxyapatite (HA) particles. Cell counting kit-8 method was used to evaluate the cytotoxicity of Ta and HA particles. The apoptosis effects were evaluated by flow cytometry, and the protein expression levels of interleukin-6 (IL-6) and tumor necrosis factor- (TNF-) were evaluated by ELISA. The protein levels of inflammation-related signaling pathways including nuclear factor-kappa B (NF-B) and extracellular regulated kinase (ERK) were detected by western blotting. The cytotoxicity test showed that the toxicity level of Ta was grade l, which is within the clinically acceptable range. Compared with the HA control, Ta had no significant effect on THP-1 cell apoptosis, IL-6, and TNF- release. The phosphorylated levels of NF-B and ERK at 3 h in the Ta group were lower than those in the HA and control groups ( < 0.001 both). These results reveal Ta particles behave good biosafety properties and provide some new insights for the future clinical use of Ta.
本研究旨在评估钽（Ta）颗粒的生物安全性，并进一步探讨 Ta 颗粒对人单核细胞毒性和炎症细胞因子表达的影响。用 Ta 和羟基磷灰石（HA）颗粒培养人单核白血病（THP-1）细胞。用细胞计数试剂盒-8 法评估 Ta 和 HA 颗粒的细胞毒性。通过流式细胞术评估细胞凋亡作用，通过 ELISA 评估白细胞介素-6（IL-6）和肿瘤坏死因子-（TNF-）的蛋白表达水平。通过 Western blot 检测炎症相关信号通路（包括核因子-kappa B（NF-B）和细胞外调节激酶（ERK））的蛋白水平。细胞毒性试验表明，Ta 的毒性水平为 1 级，在临床可接受范围内。与 HA 对照组相比，Ta 对 THP-1 细胞凋亡、IL-6 和 TNF-的释放没有显著影响。在 Ta 组中，NF-B 和 ERK 的磷酸化水平在 3 小时时均低于 HA 组和对照组（均<0.001）。这些结果表明 Ta 颗粒具有良好的生物安全性，并为 Ta 的未来临床应用提供了一些新的见解。
