Allicock Orchid M, Petrone Mary E, Yolda-Carr Devyn, Breban Mallery, Walsh Hannah, Watkins Anne E, Rothman Jessica E, Farhadian Shelli F, Grubaugh Nathan D, Wyllie Anne L
Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA.
Department of Medicine, Section of Infectious Diseases, Yale School of Medicine, New Haven, CT, 06510, USA.
medRxiv. 2021 Jul 3:2021.02.01.21250946. doi: 10.1101/2021.02.01.21250946.
There is an urgent need to expand testing for SARS-CoV-2 and other respiratory pathogens as the global community struggles to control the COVID-19 pandemic. Current diagnostic methods can be affected by supply chain bottlenecks and require the assistance of medical professionals, impeding the implementation of large-scale testing. Self-collection of saliva may solve these problems, as it can be completed without specialized training and uses generic materials. In this study, we observed thirty individuals who self-collected saliva using four different collection devices and analyzed their feedback. Two of these devices, a funnel and bulb pipette, were used to evaluate at-home saliva collection by 60 individuals. All devices enabled the safe, unsupervised self-collection of saliva. The quantity and quality of the samples received were acceptable for SARS-CoV-2 diagnostic testing, as determined by RNase P detection. Here, we demonstrate inexpensive, generic, buffer free collection devices suitable for unsupervised and home saliva self-collection.
随着全球社会努力控制新冠疫情，迫切需要扩大对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）和其他呼吸道病原体的检测。当前的诊断方法可能会受到供应链瓶颈的影响，并且需要医学专业人员的协助，这阻碍了大规模检测的实施。自行采集唾液可能会解决这些问题，因为它无需专门培训即可完成，并且使用的是通用材料。在本研究中，我们观察了30名使用四种不同采集装置自行采集唾液的个体，并分析了他们的反馈。其中两种装置，即漏斗和移液管，被用于评估60名个体在家中自行采集唾液的情况。所有装置均能实现安全、无需监督的唾液自行采集。通过核糖核酸酶P检测确定，所采集样本的数量和质量对于SARS-CoV-2诊断检测而言是可接受的。在此，我们展示了适用于无需监督和在家自行采集唾液的廉价、通用、无缓冲液的采集装置。
