Chemistry Department, Science and Letters Faculty, Adiyaman University, 02040, Adıyaman, Turkey.
Bull Environ Contam Toxicol. 2021 Apr;106(4):647-651. doi: 10.1007/s00128-021-03136-4. Epub 2021 Feb 9.
In this study, glutathione reductase (GR) from baker's yeast (Saccharomyces cerevisiae) was exposed to 0, 25, 50, 100, 250 and 500 mg/L copper(II) oxide nanoparticles (CuO NPs) and copper(II) chloride (CuCl). Changes in GR% activity upon exposure to 25, 50, 100, 250 and 500 mg/L CuO NPs and CuCl were found to be + 0.3, - 3.4, - 8.1, - 25.7 and - 37.4 and - 60.7, - 72.7, - 77.8, - 85.3 and - 90.6, respectively. The 50% inhibition concentration (IC50) was 625 ppm (78.6 × 10 M) for CuO NPs and 21 ppm (1.56 × 10 M) for CuCl. Moreover, CuO NPs and CuCl inhibited GR competitively and noncompetitively, respectively.
在这项研究中，面包酵母（酿酒酵母）中的谷胱甘肽还原酶（GR）分别暴露于 0、25、50、100、250 和 500mg/L 的氧化铜纳米颗粒（CuO NPs）和氯化铜（CuCl）中。结果发现，暴露于 25、50、100、250 和 500mg/L 的 CuO NPs 和 CuCl 后，GR%活性分别增加了+0.3、-3.4、-8.1、-25.7 和-37.4 和-60.7、-72.7、-77.8、-85.3 和-90.6。CuO NPs 的 50%抑制浓度（IC50）为 625ppm（78.6×10M），CuCl 的 IC50 为 21ppm（1.56×10M）。此外，CuO NPs 和 CuCl 分别竞争性和非竞争性地抑制了 GR。
