通过质量碎片谱法测定大鼠脑特定区域中吗啡与剂量相关的差异蓄积。

Dose-related differential accumulation of morphine in specific regions of rat brain determined by mass fragmentography.

作者信息

Fuller G N, Lin S N, Caprioli R M, Wiggins R C, Dafny N

机构信息

Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77025.

出版信息

Int J Neurosci. 1988 Jan;38(1-2):31-8. doi: 10.3109/00207458809000479.

DOI:10.3109/00207458809000479

PMID:3356502

Abstract

Regional morphine accumulation was examined in 8 brain areas (cerebral cortex, hippocampus, striatum, midbrain, hypothalamus, thalamus, medulla oblongata and cerebellum) following either a single dose or incremental doses administered by intraperitoneal injection. Morphine levels were determined by gas chromatography-mass spectrometry with chemical ionization detection. Prior to morphine assay, the vasculature was cleared of blood by saline perfusion to eliminate distortion of tissue-morphine by blood-morphine. Results indicate a dose-dependent, differential accumulation of morphine in different brain regions following incremental morphine administration. Three distinct uptake profiles were obtained, with the cerebellum, hippocampus, medulla and cortex showing roughly linear accumulation the midbrain, striatum and thalamus showing nonlinear accumulation, and the hypothalamus showing significantly higher absolute morphine levels than any other brain region.

摘要

通过腹腔注射给予单剂量或递增剂量吗啡后，对8个脑区（大脑皮层、海马体、纹状体、中脑、下丘脑、丘脑、延髓和小脑）的局部吗啡蓄积情况进行了研究。吗啡水平通过带有化学电离检测的气相色谱-质谱法测定。在进行吗啡检测之前，通过盐水灌注清除脉管系统中的血液，以消除血液中的吗啡对组织吗啡的干扰。结果表明，递增给予吗啡后，不同脑区的吗啡蓄积呈剂量依赖性差异。获得了三种不同的摄取模式，小脑、海马体、延髓和皮层呈现大致线性蓄积，中脑、纹状体和丘脑呈现非线性蓄积，而下丘脑的吗啡绝对水平显著高于其他任何脑区。