使君子水提取物逆转了在 Wistar 大鼠中由棉籽油提取物引起的睾丸损伤。
Aqueous Extract of Adansonia digitata reversed Cotton Seed Extract-Induced Testicular Damage in Wistar rats.
机构信息
Department of Anatomy, Osun State University, Osogbo, Nigeria.
Laboratory for Reproductive Biology and Developmental Programming, Department of Physiology, Edo University Iyamho, Edo State, Nigeria.
出版信息
JBRA Assist Reprod. 2021 Apr 27;25(2):257-265. doi: 10.5935/1518-0557.20200092.
OBJECTIVE
Infertility is the inability of sexually active couples without using birth control to get pregnant after one year of uninterrupted sexual intercourse. Cotton Seed Extract (CSE) has been linked to male infertility by causing oxidative damage to the testes due to the action of its active component, Gossypol. Adansonia digitata has been known to have many medically useful properties, including antioxidant effects. This study aimed at evaluating the effects of Adansonia digitata on Cottonseed extract-induced testicular damage.
METHODS
Forty (40) Adult male Wistar rats were divided into 8 groups of five rats per group (n=5). Group 1 served as the control and received 0.5 ml of phosphate buffer orally; Group 2 received 800 mg/kg b.wt A. digitata orally; Group 3 received 300 mg/kg b.wt Vitamin E only orally; Group 4 received 60 mg/kg b.wt CSE intraperitoneally; Group 5 received 20 mg/kg b.wt CSE intraperitoneally; Group 6 received 60 mg/kg b.wt CSE intraperitoneally and 800 mg/kg b.wt A. digitata orally; Group 7 received 20 mg/kg b.wt CSE intraperitoneally and 800 mg/kg b.wt A. digitata orally; Group 8 received 60 mg/kg b.wt CSE intraperitoneally and 300 mg/kg Vit. E orally. It was administered for 21 days. The testes and epididymis were dissected following abdominal incision. The epididymis was used for semen analysis while the testes was processed for histological analysis and biochemical assay. All the data was analyzed by ANOVA, using the SPSS version 17.0 software. A p<0.05 was considered significant.
RESULTS
CSE administration caused significant (p<0.05) decrease in sperm count, found in the group treated with CSE only. However, the Administration of A. digitata caused significant increase (p<0.05) in sperm count, G6PDH, LDH, GPx and SOD; however, MDA levels were decreased. Histological observations showed a decrease in the number of Spermatogonia and differentiating cells in the testes of rats treated with CSE.
CONCLUSIONS
The results obtained revealed the antioxidant ability of A. digitata in counter-acting the testicular damage caused by CSE administration.
目的
不孕是指在没有使用避孕措施的情况下，有性生活的夫妇在一年无间断的性生活后仍无法怀孕。棉籽油提取物（CSE）由于其活性成分棉酚的作用，导致睾丸氧化损伤，与男性不育有关。猴面包树已被证明具有许多医学上有用的特性，包括抗氧化作用。本研究旨在评估猴面包树对棉籽油提取物引起的睾丸损伤的影响。
方法
将 40 只成年雄性 Wistar 大鼠分为 8 组，每组 5 只（n=5）。第 1 组为对照组，给予 0.5ml 磷酸盐缓冲液口服；第 2 组给予 800mg/kg b.wt 猴面包树口服；第 3 组仅给予 300mg/kg b.wt 维生素 E 口服；第 4 组给予 60mg/kg b.wt CSE 腹腔内注射；第 5 组给予 20mg/kg b.wt CSE 腹腔内注射；第 6 组给予 60mg/kg b.wt CSE 腹腔内注射和 800mg/kg b.wt 猴面包树口服；第 7 组给予 20mg/kg b.wt CSE 腹腔内注射和 800mg/kg b.wt 猴面包树口服；第 8 组给予 60mg/kg b.wt CSE 腹腔内注射和 300mg/kg Vit.E 口服。给药 21 天。剖腹后取出睾丸和附睾。附睾用于精液分析，睾丸用于组织学分析和生化测定。所有数据均采用 SPSS 版本 17.0 软件进行方差分析。p<0.05 为差异有统计学意义。
结果
CSE 给药导致仅接受 CSE 治疗的组精子计数显著降低（p<0.05）。然而，猴面包树的给药导致精子计数、G6PDH、LDH、GPx 和 SOD显著增加（p<0.05），而 MDA 水平降低。组织学观察显示，CSE 给药大鼠睾丸中精原细胞和分化细胞数量减少。
结论
结果表明，猴面包树具有抗氧化能力，可对抗 CSE 给药引起的睾丸损伤。
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