水提物可预防氯化镉诱导的 Wistar 大鼠睾丸损伤。

Aqueous extract of prevents Cadmium Chloride-induced testicular damage in Wistar Rats.

机构信息

Department of Anatomy, Osun State University, Osogbo, Nigeria.

Laboratory for Reproductive Biology and Developmental Programming, Department of Physiology, Edo State University, Uzairue, Edo State, Nigeria.

出版信息

J Basic Clin Physiol Pharmacol. 2021 Mar 11;33(3):347-353. doi: 10.1515/jbcpp-2020-0222.

DOI:10.1515/jbcpp-2020-0222

PMID:33705611

Abstract

OBJECTIVES

The role of aqueous extract of was investigated against cadmium chloride-induced testicular damage in Wistar Rats.

METHODS

Thirty (30) male Wistar Rats weighing (150-170) were divided into six groups (n=5). Group A served as control and received oral administration of phosphate buffer saline; group B received 800 mg/kg only; group C were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride; group D were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride and treated with 800 mg/kg aqueous extract of ; group E received 300 mg/kg vitamin E only; group F were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride and treated with 300 mg/kg vitamin E. After 21 days, the animals were sacrificed by cervical dislocation, the testes were excised fixed in Bouins fluids for histological analysis and the other homogenized in 5% sucrose solution for determination of tissue malondialdehyde (MDA) and antioxidant enzyme activity, biochemical assay.

RESULTS

The group treated with cadmium chloride plus caused significant decrease in MDA levels with significant increase (p<0.05) in antioxidant activities and biochemical enzymes when compared to cadmium chloride only group.

CONCLUSIONS

Aqueous extract of appears to have ameliorative effect against cadmium chloride-induced testicular damage. This could be attributed to the presence of polyphenolic compound.

摘要

目的

研究水提物对氯化镉诱导的 Wistar 大鼠睾丸损伤的作用。

方法

将 30 只雄性 Wistar 大鼠（体重 150-170 克）分为 6 组（每组 5 只）。A 组为对照组，给予磷酸盐缓冲液口服；B 组给予 800mg/kg 水提物；C 组腹腔注射单次 2mg/kg 氯化镉；D 组腹腔注射单次 2mg/kg 氯化镉，同时给予 800mg/kg 水提物；E 组给予 300mg/kg 维生素 E；F 组腹腔注射单次 2mg/kg 氯化镉，同时给予 300mg/kg 维生素 E。21 天后，通过颈椎脱位处死动物，切除睾丸，用 Bouins 液固定进行组织学分析，另将其匀浆于 5%蔗糖溶液中，用于测定组织丙二醛（MDA）和抗氧化酶活性的生化分析。