Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China.
Department of Pulmonology, Southwest Hospital, Army Medical University, Chongqing 400038, PR China.
Per Med. 2021 Mar;18(2):129-139. doi: 10.2217/pme-2020-0019. Epub 2021 Feb 10.
To explore the role of urine cell-free DNA (ucfDNA) concentration and integrity indexes as potential biomarkers for lung cancer diagnosis. Quantitative real-time PCR targeting Arthrobacter luteus () repeats at three size fragments (-60, 115 and 247 bp) was performed in 55 lung cancer patients and 35 healthy individuals. ucfDNA concentration and integrity indexes were significantly higher in lung cancer patients than in healthy controls. The area under the receiver operating characteristic curve for differentiating patients with stage I/II from healthy controls by fragments concentration were 0.856, 0.909 and 0.932, respectively. In addition, the ucfDNA integrity indexes in patients with lymph node metastasis were significantly higher than in patients with non-metastatic. ucfDNA concentration and integrity indexes could serve as promising biomarkers for lung cancer diagnosis.
探讨尿游离 DNA（ucfDNA）浓度和完整性指标作为肺癌诊断潜在生物标志物的作用。
采用针对节杆菌（Arthrobacter luteus）三个大小片段（-60、115 和 247bp）的实时定量 PCR 技术，对 55 例肺癌患者和 35 例健康个体进行检测。
肺癌患者的 ucfDNA 浓度和完整性指标明显高于健康对照组。通过三条片段浓度区分 I/II 期患者与健康对照组的受试者工作特征曲线下面积分别为 0.856、0.909 和 0.932。此外，有淋巴结转移的患者的 ucfDNA 完整性指标明显高于无转移的患者。
ucfDNA 浓度和完整性指标可作为肺癌诊断的有前途的生物标志物。
