Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan 063000, PR China.
Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai 200231, PR China.
Per Med. 2021 Mar;18(2):97-106. doi: 10.2217/pme-2020-0027. Epub 2021 Feb 10.
This study explored the potential function of miR-452-5p in hepatocellular carcinoma (HCC) and clarified the mechanism underlying HCC progression. Real-time quantitative PCR was used to detect miR-452-5p and COLEC10 mRNA expression in HCC, western blot was performed to test COLEC10 protein expression. The regulatory mechanism of miR-452-5p/COLEC10 in HCC cells was explored using CCK-8, wound healing assay, Transwell and dual-luciferase reporter assay. MiR-452-5p was greatly upregulated in HCC cells, and it served as an oncogene playing an active role in HCC cell proliferation, migration and invasion. COLEC10 was identified as the target of miR-452-5p in HCC attenuating the promoting effect of miR-452-5p on HCC cells upon overexpression. MiR-452-5p can promote the progression of HCC via targeting COLEC10.
本研究旨在探讨 miR-452-5p 在肝细胞癌（HCC）中的潜在功能，并阐明 HCC 进展的机制。实时定量 PCR 用于检测 HCC 中 miR-452-5p 和 COLEC10 mRNA 的表达，Western blot 用于检测 COLEC10 蛋白的表达。通过 CCK-8、划痕愈合实验、Transwell 和双荧光素酶报告基因实验探索 miR-452-5p/COLEC10 在 HCC 细胞中的调控机制。miR-452-5p 在 HCC 细胞中显著上调，作为一种癌基因，在 HCC 细胞增殖、迁移和侵袭中发挥积极作用。COLEC10 被鉴定为 HCC 中 miR-452-5p 的靶基因，可减弱过表达 miR-452-5p 对 HCC 细胞的促进作用。miR-452-5p 可通过靶向 COLEC10 促进 HCC 的进展。
