Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmacodynamics and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Saudi J Kidney Dis Transpl. 2020 Nov-Dec;31(6):1310-1319. doi: 10.4103/1319-2442.308340.
Diazinon (DZN) is an organophosphate pesticide that is commonly used in agriculture worldwide, including in Iran, and unfortunately, it leads to a variety of negative effects on the environment, animals, and humans. Alpha-lipoic acid (ALA) is an antioxidant agent that acts via scavenging of oxygen-free radicals. Collagen IV is a component of the main base membrane structure and DZN may also affect the expression of this key protein. The aim of this study was to evaluate antioxidant properties of ALA on the expression of collagen IV, renal function, and oxidative stress induced by DZN in renal tissue. In this experimental study, 30 adult male Wistar rats were randomly divided into five groups (n = 6) including: the control group, DZN (40 mg/kg) group, ALA (100 mg/kg) group, ALA (100 mg/kg) + DZN (40 mg/kg) group, and sham group. On day 0 and after 6 weeks, the urine and blood samples were collected to measure glomerular filtration rate (GFR). After 6 weeks, the rats were anesthetized and the left kidney was used for immunohistochemistry study and the right kidney was used to evaluate the oxidative stress parameters. The results have shown that ALA significantly improved the biochemical parameters including superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, and GFR. In addition, ALA significantly prevented the expression of collagen IV that was changed by DZN administration in rats. We concluded that when exposed to DZN, depletion of antioxidant enzymes is accompanied by the induction of oxidative stress that might be beneficial in monitoring DZN toxicity and alpha-lipoic acid, as an antioxidant can overcome the toxicity induced by DZN in the kidney.
敌敌畏（DZN）是一种有机磷农药，在全世界范围内广泛用于农业，包括伊朗。不幸的是，它会对环境、动物和人类造成多种负面影响。α-硫辛酸（ALA）是一种抗氧化剂，通过清除氧自由基发挥作用。胶原 IV 是主要基底膜结构的组成部分，DZN 也可能影响这种关键蛋白质的表达。本研究旨在评估 ALA 的抗氧化特性对胶原 IV 表达、肾功能和 DZN 诱导的肾组织氧化应激的影响。在这项实验研究中，30 只成年雄性 Wistar 大鼠被随机分为五组（n = 6），包括：对照组、DZN（40mg/kg）组、ALA（100mg/kg）组、ALA（100mg/kg）+DZN（40mg/kg）组和假手术组。在第 0 天和 6 周后，收集尿液和血液样本以测量肾小球滤过率（GFR）。6 周后，麻醉大鼠，使用左肾进行免疫组织化学研究，使用右肾评估氧化应激参数。结果表明，ALA 显著改善了超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶、谷胱甘肽还原酶和 GFR 等生化参数。此外，ALA 显著阻止了 DZN 给药引起的胶原 IV 表达的改变。我们得出结论，当暴露于 DZN 时，抗氧化酶的耗竭伴随着氧化应激的诱导，这可能有助于监测 DZN 毒性和α-硫辛酸，因为抗氧化剂可以克服 DZN 在肾脏中的毒性。
