Student Research Committee, Department of Pharmacology and Toxicology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Drug Chem Toxicol. 2019 Nov;42(6):585-591. doi: 10.1080/01480545.2018.1454459. Epub 2018 Apr 12.
Several studies have shown that oxidative stress and cell damage can occur at very early stages of diazinon (DZN) exposure. The present study was designed to determine the beneficial effect of thymoquinone (Thy), the main component of (black seed or black cumin), against DZN cardio-toxicity in rats. In the present experimental study, 48 male Wistar rats were randomly divided into six groups: control (corn oil gavages), DZN gavages (20 mg/kg/day), Thy gavages (10 mg/kg/day) and Thy + DVN gavages (2.5, 5 and 10 mg/kg/day). Treatments were continued for 28 days, then the animals were anesthetized by ether and superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), lactate dehydrogenize (LDH) and glutathione peroxide (GPX) activity was evaluated. In addition, glutathione (GSH) and malondialdehyde (MDA) the heart tissue and creatinephosphokinase-MB (CPK-MB) and troponin (TPI) levels and cholinesterase activity in the blood were evaluated. DZN-induced oxidative damage and elevated the levels of the cardiac markers CK-MB, TPI, MDA and LDH and decreased SOD, CAT and cholinesterase activity and GSH level compared with the control group. Treatment with Thy reduced DZN cardio-toxicity and cholinesterase activity. The success of Thy supplementation against DZN toxicity can be attributed to the antioxidant effects of its constituents. Administration of Thy as a natural antioxidant decreased DZN cardio-toxicity and improved cholinesterase activity in rats through the mechanism of free radical scavenging.
几项研究表明，氧化应激和细胞损伤可能在敌敌畏（DZN）暴露的早期阶段发生。本研究旨在确定百里醌（Thy），即（黑种子或黑孜然）的主要成分，对大鼠敌敌畏心脏毒性的有益作用。在本实验研究中，将 48 只雄性 Wistar 大鼠随机分为六组：对照组（玉米油灌胃）、DZN 灌胃组（20mg/kg/天）、Thy 灌胃组（10mg/kg/天）和 Thy+DZN 灌胃组（2.5、5 和 10mg/kg/天）。治疗持续 28 天，然后用乙醚麻醉动物，评估超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽 S-转移酶（GST）、乳酸脱氢酶（LDH）和谷胱甘肽过氧化物酶（GPX）的活性。此外，还评估了心脏组织中的谷胱甘肽（GSH）和丙二醛（MDA）以及血液中的肌酸磷酸激酶-MB（CPK-MB）和肌钙蛋白（TPI）水平和胆碱酯酶活性。与对照组相比，DZN 诱导的氧化损伤增加了心脏标志物 CK-MB、TPI、MDA 和 LDH 的水平，并降低了 SOD、CAT 和胆碱酯酶活性以及 GSH 水平。Thy 治疗减轻了 DZN 的心脏毒性和胆碱酯酶活性。Thy 补充剂对抗 DZN 毒性的成功可以归因于其成分的抗氧化作用。通过清除自由基的机制，Thy 的给药作为一种天然抗氧化剂，降低了 DZN 对大鼠的心脏毒性并改善了胆碱酯酶活性。
