Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
Department of Structural and Functional Biology, Institute of Biociences, State University of Sao Paulo, Botucatu, Brazil.
Reprod Domest Anim. 2021 May;56(5):754-763. doi: 10.1111/rda.13914. Epub 2021 Mar 8.
In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus-oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production.
与体内相比，体外胚胎生产（IVP）的效率降低。牛体外卵母细胞成熟（IVM）机制的基础知识为提高体外胚胎生产产量提供了支持。本研究评估了骨形态发生蛋白 15（BMP15）、成纤维细胞生长因子 16（FGF16）及其联合作用对牛体外成熟卵母细胞（COC）扩张、卵母细胞和 CC 碎片、卵母细胞核成熟、能量代谢和孕激素产生的影响。在对照或补充培养基中培养 COC，含有 BMP15（100ng/ml）、FGF16（10ng/ml）或 BMP15 与 FGF16 联合；并在 IVM 的 0 和 22 小时进行评估。BMP15 单独或与 FGF16 联合可促进卵丘扩展。BMP15 降低了 CC 和卵母细胞中的 DNA 碎片化，并提高了卵母细胞核成熟率。此外，BMP15 增加了 CC 孕激素的产生，这是以前没有报道过的。本研究加强了先前的数据，表明 BMP15 在 IVM 期间具有有益的影响，同时提供了新的证据，表明潜在的机制涉及增加孕激素的产生。
