Machado Mariana Fernandes, Caixeta Ester Siqueira, Sudiman Jaqueline, Gilchrist Robert B, Thompson Jeremy G, Lima Paula Fernanda, Price Christopher A, Buratini José
Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista, São Paulo, Brazil.
The Robinson Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, Adelaide, South Australia, Australia.
Theriogenology. 2015 Aug;84(3):390-8. doi: 10.1016/j.theriogenology.2015.03.031. Epub 2015 Apr 1.
Bone morphogenetic protein 15 (BMP15) and members of the fibroblast growth factor (FGF) family are expressed by the oocyte and are involved in the control of cumulus cell function. We tested the hypothesis that FGF17, alone or combined with BMP15 in the maturation medium, enhances cumulus expansion, meiosis progression, embryonic development, and expression of mRNA encoding key genes regulating expansion (prostaglandin-endoperoxide synthase 2 [PTGS2], hyaluronan synthase 2 [HAS2], tumor necrosis factor-stimulated gene 6 [TNFAIP6], and pentraxin 3 [PTX3]) and markers of oocyte developmental competence (phosphofructokinase [PFKP], gremlin [GREM1], versican [VCAN], and the genomic progesterone receptor [nPR]) in cumulus cells. Fibroblast growth factor 17 and BMP15 increased the percentage of fully expanded cumulus-oocyte complexes (COCs), but there was no additive effect when both were combined. Neither FGF17 nor BMP15 altered the percentage of oocytes reaching meiosis II at the end of COC culture or cleavage and blastocyst rates after IVF. However, embryo quality, as assessed by the number of cells in the inner cell mass, was improved by the combination of FGF17 with BMP15. Fibroblast growth factor 17 alone did not alter gene expression in cumulus cells at the end of IVM, whereas BMP15 increased PTGS2 and PTX3 mRNA levels. The combination of FGF17 and BMP15 increased nPR mRNA abundance in cumulus cells but did not change the expression of other markers of developmental competence. This study provides novel evidence that FGF17 enhances cumulus expansion in bovine COCs submitted to IVM and that the supplementation of the IVM medium with FGF17 and BMP15 may improve embryo quality.
骨形态发生蛋白15（BMP15）和成纤维细胞生长因子（FGF）家族成员由卵母细胞表达，并参与对卵丘细胞功能的调控。我们检验了以下假设：在成熟培养基中，单独的FGF17或与BMP15联合使用，可增强卵丘扩展、减数分裂进程、胚胎发育，以及编码调控扩展的关键基因（前列腺素内过氧化物合酶2 [PTGS2]、透明质酸合酶2 [HAS2]、肿瘤坏死因子刺激基因6 [TNFAIP6]和五聚素3 [PTX3]）和卵丘细胞中卵母细胞发育能力标志物（磷酸果糖激酶[PFKP]、gremlin [GREM1]、多功能蛋白聚糖[VCAN]和基因组孕酮受体[nPR]）的mRNA表达。FGF17和BMP15增加了完全扩展的卵丘-卵母细胞复合体（COC）的百分比，但两者联合使用时没有相加效应。FGF17和BMP15均未改变COC培养结束时达到减数分裂II期的卵母细胞百分比，或体外受精后的卵裂率和囊胚率。然而，通过内细胞团中的细胞数量评估，FGF17与BMP15联合使用可提高胚胎质量。单独的FGF17在体外成熟结束时未改变卵丘细胞中的基因表达，而BMP15增加了PTGS2和PTX3 mRNA水平。FGF17和BMP15联合使用增加了卵丘细胞中nPR mRNA丰度，但未改变其他发育能力标志物的表达。本研究提供了新的证据，表明FGF17可增强体外成熟的牛COC中的卵丘扩展，并且在体外成熟培养基中添加FGF17和BMP15可能会提高胚胎质量。
