Chang Chin-Feng, Huang Sing-Yii, Lee Ching-Fu
Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan, ROC.
Department of Applied Science, National Tsing Hua University, Nanda Campus, Hsinchu 30014, Taiwan, ROC.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004703. Epub 2021 Feb 10.
Mangroves grow in the intertidal zone, which alternates between fresh water and sea water, with abundant bioresources. In mangrove habitats, yeasts play an important role in the decomposition of organic matter, and such nutrient cycling has high ecological importance. During a study of the diversity of yeast from Taiwan mangroves, seven strains of basidiomycetous yeasts were isolated and these yeasts represent two novel yeast species belonging to the genus . According to the sequences of the D1/D2 domain of large subunit (LSU) rRNA and the internal transcribed spacer (ITS) region, these seven strains could be clearly classified into two groups representing two individual, distinct species. Strains HM5L06, HM6L07, HM11L11 and BJ3S01, differed from their closest relative species by 4-5 nt substitutions (no gaps) in the sequences of the D1/D2 domain of LSU rRNA and by 23 nt substitutions (10 gaps) in the ITS region. Strains HM6L11, HM7L02 and HM8L19, differed from their closest relative species by 7 nt substitutions (one gap) in the sequences of the D1/D2 domain of LSU rRNA and by 27 nt substitutions (12 gaps) in the ITS region. The scientific names of sp. nov. and sp. nov. are proposed for these strains. The holotypes are BCRC 23477 (ex-type HM5L06=CBS 16558; MycoBank number MB837428) and BCRC 23478 (ex-type HM6L11=CBS 16556; MycoBank number MB837429).
红树林生长在潮间带，这里淡水与海水交替出现，生物资源丰富。在红树林栖息地，酵母在有机物分解过程中发挥着重要作用，这种营养循环具有很高的生态重要性。在一项对台湾红树林酵母多样性的研究中，分离出了七株担子菌酵母，这些酵母代表了属于该属的两个新酵母物种。根据大亚基（LSU）rRNA的D1/D2结构域和内部转录间隔区（ITS）区域的序列，这七株菌株可明确分为两组，代表两个独立、不同的物种。菌株HM5L06、HM6L07、HM11L11和BJ3S01，在LSU rRNA的D1/D2结构域序列中与其最相近的相关物种有4 - 5个核苷酸替换（无缺口），在ITS区域有23个核苷酸替换（10个缺口）。菌株HM6L11、HM7L02和HM8L19，在LSU rRNA的D1/D2结构域序列中与其最相近的相关物种有7个核苷酸替换（1个缺口），在ITS区域有27个核苷酸替换（12个缺口）。为这些菌株提出了新物种和新物种的学名。模式菌株分别是BCRC 23477（模式菌株HM5L06 = CBS 16558；国际真菌命名数据库编号MB837428）和BCRC 23478（模式菌株HM6L11 = CBS 16556；国际真菌命名数据库编号MB837429）。
