Facultad de Medicina Humana, Universidad Científica del Sur, Lima, Perú.
Instituto Nacional Materno Perinatal, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2020 Oct-Dec;37(4):716-720. doi: 10.17843/rpmesp.2020.374.5422. Epub 2021 Feb 3.
The objective of this study was to evaluate the presence of colistin- and carbapenemic-resistant genes in multidrug-resistant Klebsiella pneumoniae strains isolated at the Instituto Materno Perinatal de Lima (2015-2018). Susceptibility levels were analyzed by disk diffusion and microdilution. The presence of colistin- and carbapenemic-resistant genes was determined by polymerase chain reaction (PCR) and was related to clonality. A total of 36 K. pneumoniae strains were analyzed, 5 (13.8%) were resistant to colistin and belonged to different clonal groups. Only 2 strains were found with carbapenemases (bla KPC and bla NDM), and no colistin plasmid genes were detected. High resistance levels to the other tested antimicrobials were observed, except for amikacin (13.9%). The results highlight the presence of colistin-resistant strains (33.3% in 2018), a worrying situation as they are part of the latest treatment alternatives for infections caused by multiresistant pathogens.
本研究旨在评估 2015-2018 年利马母胎研究所（Instituto Materno Perinatal de Lima）分离的多药耐药肺炎克雷伯菌（Klebsiella pneumoniae）菌株中是否存在粘菌素和碳青霉烯类耐药基因。通过纸片扩散法和微量稀释法分析药敏水平。通过聚合酶链反应（PCR）检测是否存在粘菌素和碳青霉烯类耐药基因，并与克隆性相关。共分析了 36 株肺炎克雷伯菌，5 株（13.8%）对粘菌素耐药，属于不同的克隆群。仅发现 2 株带有碳青霉烯酶（bla KPC 和 bla NDM），未检测到粘菌素质粒基因。除阿米卡星（13.9%）外，其他测试的抗菌药物的耐药水平较高。结果表明存在耐粘菌素菌株（2018 年为 33.3%），这是一个令人担忧的情况，因为它们是治疗多药耐药病原体引起感染的最新治疗选择的一部分。
