Wang Zhoutao, Ren Hui, Xu Fu, Lu Guilong, Cheng Wei, Que Youxiong, Xu Liping
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Research Centre of National Sugarcane Engineering Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2021 Feb 8;10(2):322. doi: 10.3390/plants10020322.
Sugarcane is an important sugar and bioenergy ethanol crop, and the hyperploidy has led to stagnant progress in sugarcane genome decipherment, which also hindered the genome-wide analyses of versatile lectin receptor kinases (LecRKs). The published genome of , one of the two sugarcane ancestor species, enables us to study the characterization of LecRKs and their responses to sugarcane leaf blight (SLB) triggered by . A total of 429 allelic and non-allelic LecRKs, which were classified into evolved independently three types according to signal domains and phylogeny, were identified based on the genome. Regarding those closely related LecRKs in the phylogenetic tree, their motifs and exon architectures of representative L- and G-types were similar or identical. LecRKs showed an unequal distribution on chromosomes and more G-type tandem repeats may come from the gene expansion. Comparing the differentially expressed LecRKs (DELs) in response to SLB in sugarcane hybrid and ancestor species , we found that the DEL number in the shared gene sets was highly variable among each sugarcane accession, which indicated that the expression dynamics of LecRKs in response to SLB were quite different between hybrids and particularly between sugarcane hybrid and . In addition, C-type LecRKs may participate in metabolic processes of plant-pathogen interaction, mainly including pathogenicity and plant resistance, indicating their putative roles in sugarcane responses to SLB infection. The present study provides a basic reference and global insight into the further study and utilization of LecRKs in plants.
甘蔗是一种重要的糖料和生物能源乙醇作物，超倍性导致甘蔗基因组解析进展停滞，这也阻碍了对多功能凝集素受体激酶（LecRKs）的全基因组分析。已发表的甘蔗两个祖先物种之一的基因组，使我们能够研究LecRKs的特征及其对由[病原体名称缺失]引发的甘蔗叶枯病（SLB）的反应。基于该基因组，共鉴定出429个等位基因和非等位基因的LecRKs，根据信号域和系统发育将它们独立地分为三种类型。对于系统发育树中那些密切相关的LecRKs，其代表性L型和G型的基序和外显子结构相似或相同。LecRKs在染色体上分布不均，更多的G型串联重复可能来自基因扩增。比较甘蔗杂交种和祖先物种[物种名称缺失]中对SLB有反应的差异表达LecRKs（DELs），我们发现共享基因集中的DEL数量在每个甘蔗品种中差异很大，这表明LecRKs对SLB的表达动态在杂交种之间，特别是在甘蔗杂交种和[物种名称缺失]之间有很大不同。此外，C型LecRKs可能参与植物-病原体相互作用的代谢过程，主要包括致病性和植物抗性，表明它们在甘蔗对SLB感染的反应中可能发挥的作用。本研究为进一步研究和利用植物中的LecRKs提供了基本参考和全面见解。
