肽-核苷类抗生素的光电二极管阵列检测
Photodiode array detection of peptide-nucleoside antibiotics.
作者信息
Mierzwa R, Cooper R, Pramanik B
机构信息
Pharmaceutical Research Division, Schering-Plough Corporation, Bloomfield, NJ 07003.
出版信息
J Chromatogr. 1988 Feb 5;436(2):259-67. doi: 10.1016/s0021-9673(00)94583-7.
The discrimination within fermentation matrices of some structurally related peptide-nucleosides is vastly simplified by coupling photodiode array detection to high-performance liquid chromatography (HPLC). Blasticidin S analogues and other closely related nucleosides can easily be discriminated with on-line photodiode array spectral acquisition (PDA). From the combined HPLC-PDA evaluation of the fermentation filtrate from culture broth 83-2245, two blasticidin S type compounds, Sch 36605 and Sch 36606 could be detected. Mildiomycin, a structurally related nucleoside produced from ATCC culture 31120, could be detected during preliminary isolation without an authentic reference for comparison. Fast atom bombardment mass spectroscopic evaluations and mass-analysed ion kinetic energy spectra of these nucleosides confirmed their respective identities.
通过将光电二极管阵列检测与高效液相色谱（HPLC）联用，极大地简化了在一些结构相关的肽 - 核苷发酵基质中的鉴别过程。杀稻瘟菌素S类似物和其他密切相关的核苷可以通过在线光电二极管阵列光谱采集（PDA）轻松鉴别。通过对来自83 - 2245培养液的发酵滤液进行HPLC - PDA联合评估，可以检测到两种杀稻瘟菌素S型化合物，即Sch 36605和Sch 36606。米尔迪霉素是由ATCC培养物31120产生的一种结构相关核苷，在初步分离过程中，即使没有真实对照品进行比较也能被检测到。这些核苷的快原子轰击质谱评估和质量分析离子动能谱证实了它们各自的身份。
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