Department of Biochemistry, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India.
Department of Chemical Engineering, Indian Institute of Technology, Kanpur, Uttar Pradesh 208016, India.
Comb Chem High Throughput Screen. 2022;25(10):1672-1681. doi: 10.2174/1386207324666210210112843.
Over the last few decades, there has been a stupendous change in the area of drug delivery using particulate delivery systems, with increasing focus on nanoparticles in recent times. Nanoparticles help to improve and alter the pharmacodynamic properties and pharmacokinetics of various types of drug molecules. These features help to protect the drug entity in the systemic circulation, access of the drug to the chosen sites, and to deliver the drug in a controlled and sustained rate at the site of action.
Nanoparticle based targeted delivery of anti-inflammatory drugs/signal modulatory agents to the cytoplasm or nuclei of the targeted cell can significantly enhance the precision and efficacy of intended therapeutic activity. To this end, we report ligand free, enhanced intra-nuclear delivery model of anti-inflammatory therapeutics via PDMS nanoparticles.
PDMS nanoparticles were prepared by sacrificial silica template-based approach and details of their characterization for suitability as a nanoparticle-based delivery material are detailed herein.
Biological evaluation for compatibility was carried out and the results showed that the PDMS nanoparticle has no toxicity on RAW 264.7 cells in the concentration range of 10, 20, 40, 60, 80, 100 and 120 μg/mL in culture. Biocompatibility and absence of toxicity were determined by morphological examination and cell viability assays. Drug loading and release kinetics were carried out with the anti-inflammatory drug Diclofenac.
In this paper, we clearly demonstrate the various aspects of nanoparticle articulation, characterization, effect of their characteristics and their applications as a non-toxic drug delivery molecule for its potential applications in therapeutic delivery of drugs for sustained release.
在过去的几十年中，利用微粒传递系统在药物传递领域发生了巨大的变化，最近越来越关注纳米颗粒。纳米颗粒有助于改善和改变各种类型药物分子的药效学和药代动力学特性。这些特性有助于保护药物实体在全身循环中，使药物到达选择的部位，并以受控和持续的速度在作用部位递药。
将抗炎药物/信号调节剂的基于纳米颗粒的靶向递送至靶向细胞的细胞质或细胞核中，可以显著提高预期治疗活性的精度和疗效。为此，我们报告了通过 PDMS 纳米颗粒进行无配体的、增强的抗炎治疗剂的核内递药模型。
通过牺牲二氧化硅模板法制备 PDMS 纳米颗粒，并详细介绍了其作为纳米颗粒递药材料的适用性的特征描述。
进行了相容性的生物评价，结果表明，在 10、20、40、60、80、100 和 120μg/ml 的培养浓度范围内，PDMS 纳米颗粒对 RAW 264.7 细胞没有毒性。通过形态学检查和细胞活力测定确定了生物相容性和无毒性。用抗炎药物双氯芬酸进行了载药和释放动力学研究。
在本文中，我们清楚地展示了纳米颗粒的各个方面，包括其特征化、特性的影响及其作为无毒药物传递分子的应用，以潜在地用于药物的治疗性持续释放。
