Delfani Somaye, Rezaei Faranak, Soroush Setareh, Shakib Pegah
Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khoramabad. Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad. Iran.
Recent Pat Antiinfect Drug Discov. 2021 Feb 9. doi: 10.2174/1574891X16666210210101912.
Methicillin-resistant coagulase-negative staphylococci is responsible for hospital and community-acquired infections.
This study aimed to investigate the antibiotic-resistance patterns, antibiotic-resistance genes, namely, ermA, ermB, ermC, blaZ, msrA, tetK, tetM, mup, and vanA, biofilm formation, and prevalence of different SCCmec types among the Staphylococcus cohniistrains isolated from clinical samples in Tehran, Iran.
In this study,S. cohniiisolates were screened from the clinical samples from March 2012 to February 2013 in Tehran, Iran.Antimicrobial susceptibility test and inducible clindamycin resistance were evaluated by disc diffusion method, andresistance genes were examined using Polymerase Chain Reaction (PCR) assays. Then, biofilm formation assay was analyzed by Microtiter-plate test to detect the icaA and icaDgenes. The SCCmec and the Arginine Catabolite Mobile Element (ACME) typing were performed using the PCRmethod.
FromtwentyS. cohnii, all isolates were resistant to cefoxitin. 95% of the S. cohnii was defined as multidrug resistance (MDR)strains. The ermB, ermC, and vanA genes were not detected in any isolates; however, the blaZ gene had the highest frequency.95% of the S. cohnii isolates produced biofilm. Also, 4 SCCmec types, including V, IV, III+ (C2), VIII+ (AB1), were identified. Therefore, the majority of SCCmec were untypable. Based on the ACME typing, arcA and opp3 genes were positive in 13 (65%) and 1 (5%) isolates, respectively.
Due to the high antimicrobial resistance and the spread of untypableSCCmecamong the isolates studied, the control and treatment of methicillin-resistantS. cohnii in hospitals and public health centers is a significant concern.
耐甲氧西林凝固酶阴性葡萄球菌是医院感染和社区获得性感染的病原体。
本研究旨在调查从伊朗德黑兰临床样本中分离出的科氏葡萄球菌菌株的抗生素耐药模式、抗生素耐药基因（即ermA、ermB、ermC、blaZ、msrA、tetK、tetM、mup和vanA）、生物膜形成情况以及不同SCCmec类型的流行情况。
在本研究中，从2012年3月至2013年2月在伊朗德黑兰的临床样本中筛选科氏葡萄球菌分离株。采用纸片扩散法评估抗菌药物敏感性试验和诱导型克林霉素耐药性，并使用聚合酶链反应（PCR）检测耐药基因。然后，通过微量滴定板试验分析生物膜形成试验以检测icaA和icaD基因。使用PCR方法进行SCCmec和精氨酸分解代谢移动元件（ACME）分型。
从20株科氏葡萄球菌中，所有分离株均对头孢西丁耐药。95%的科氏葡萄球菌被定义为多重耐药（MDR）菌株。在任何分离株中均未检测到ermB、ermC和vanA基因；然而，blaZ基因的频率最高。95%的科氏葡萄球菌分离株产生生物膜。此外，鉴定出4种SCCmec类型，包括V型、IV型、III +（C2）、VIII +（AB1）。因此，大多数SCCmec无法分型。基于ACME分型，arcA和opp3基因分别在13株（65%）和1株（5%）分离株中呈阳性。
由于所研究分离株中抗菌药物耐药性高且无法分型的SCCmec传播，医院和公共卫生中心对耐甲氧西林科氏葡萄球菌的控制和治疗是一个重大问题。
