Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan, 40227, ROC.
Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, 40227, ROC.
Nat Commun. 2021 Feb 10;12(1):902. doi: 10.1038/s41467-021-21229-w.
We previously found that B and AGL6 proteins form L (OAP3-2/OAGL6-2/OPI) and SP (OAP3-1/OAGL6-1/OPI) complexes to determine lip/sepal/petal identities in orchids. Here, we show that the functional L' (OAP3-1/OAGL6-2/OPI) and SP' (OAP3-2/OAGL6-1/OPI) complexes likely exist and AP3/PI/AGL6 genes have acquired additional functions during evolution. We demonstrate that the presumed L' complex changes the structure of the lower lateral sepals and helps the lips fit properly in the center of the flower. In addition, we find that OAP3-1/OAGL6-1/OPI in SP along with presumed SP' complexes regulate anthocyanin accumulation and pigmentation, whereas presumed L' along with OAP3-2/OAGL6-2/OPI in L complexes promotes red spot formation in the perianth. Furthermore, the B functional proteins OAP3-1/OPI and OAGL6-1 in the SP complex could function separately to suppress sepal/petal senescence and promote pedicel abscission, respectively. These findings expand the current knowledge behind the multifunctional evolution of the B and AGL6 genes in plants.
我们之前发现 B 和 AGL6 蛋白形成 L（OAP3-2/OAGL6-2/OPI）和 SP（OAP3-1/OAGL6-1/OPI）复合物，以决定兰花的唇瓣、萼片和花瓣的身份。在这里，我们表明功能性的 L'（OAP3-1/OAGL6-2/OPI）和 SP'（OAP3-2/OAGL6-1/OPI）复合物可能存在，并且 AP3/PI/AGL6 基因在进化过程中获得了额外的功能。我们证明，假定的 L'复合物改变了下侧萼片的结构，并帮助唇瓣恰当地位于花朵的中心。此外，我们发现 SP 中的 OAP3-1/OAGL6-1/OPI 与假定的 SP'复合物一起调节花青素的积累和色素沉着，而假定的 L'与 L 复合物中的 OAP3-2/OAGL6-2/OPI 一起促进了周皮中红点的形成。此外，SP 复合物中的 B 功能蛋白 OAP3-1/OPI 和 OAGL6-1 可以分别独立地抑制萼片/花瓣衰老并促进花梗脱落。这些发现扩展了植物中 B 和 AGL6 基因多功能进化背后的现有知识。
