Kannourakis G, Begley C G, Johnson G R, Werkmeister J A, Burns G F
Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, Australia.
J Immunol. 1988 Apr 15;140(8):2489-94.
The role of accessory cells in modulation of in vitro hemopoiesis was studied using C-mediated lysis with mAb of differing specificities. One such antibody, 25E11, which bound to all NK cells and a subset of monocytes, consistently led to enhancement of in vitro hemopoiesis when 25E11+ cells were depleted by complement or the FACS. In cultures maximally stimulated by erythropoietin and human placental conditioned medium, depletion of 25E11+ cells from beta-thalassemic PBMC resulted in up to a fourfold enhancement of multi-potential and pure erythroid colonies and a twofold enhancement of non-erythroid colonies when compared with C-only treated cells. This enhancement of in vitro hemopoiesis was also observed with cells from normal bone marrow, cord blood, and peripheral blood. The enhancement of in vitro hemopoiesis was abrogated by removal of either adherent cells or monocytes using Leu M3 antibody and C lysis. The data presented herein suggest that NK cells and a subset of monocytes may exert a negative regulatory control on both granulopoiesis and erythropoiesis. Consequently, the number of progenitor and multipotential cells in cultures of unfractionated cell populations may be greatly underestimated.
利用具有不同特异性的单克隆抗体介导的补体介导细胞裂解，研究了辅助细胞在体外造血调节中的作用。一种这样的抗体，25E11，它能与所有自然杀伤细胞（NK细胞）和一部分单核细胞结合，当通过补体或荧光激活细胞分选术（FACS）去除25E11阳性细胞时，体外造血作用持续增强。在由促红细胞生成素和人胎盘条件培养基最大程度刺激的培养物中，与仅用补体处理的细胞相比，从β地中海贫血患者外周血单个核细胞（PBMC）中去除25E11阳性细胞后，多能和纯红系集落增加了四倍，非红系集落增加了两倍。在来自正常骨髓、脐带血和外周血的细胞中也观察到了这种体外造血作用的增强。使用抗Leu M3抗体和补体介导的细胞裂解去除贴壁细胞或单核细胞后，体外造血作用的增强被消除。本文提供的数据表明，NK细胞和一部分单核细胞可能对粒细胞生成和红细胞生成发挥负调节作用。因此，未分级细胞群体培养物中祖细胞和多能细胞的数量可能被大大低估。
