Yang Jianing, Tang Jun, Li Juan, Cen Ying, Chen Junjie, Dai Gengwu
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, China.
Department of Dermatology, Sichuan Provincial People's Hospital, Chengdu, China.
Ann Transl Med. 2021 Jan;9(2):158. doi: 10.21037/atm-20-7974.
Osteosarcoma (OS) is a common bone cancer in children and adolescents which causes a large number of cancer-related deaths. Eukaryotic Translation Elongation Factor 1 Alpha 2 (EEF1A2) has been revealed to have carcinogenic properties and promote tumor progression in many cancers. We want to investigate the biological function and mechanism of EEF1A2 in OS.
The expression of EEF1A2 in OS was investigated using the Gene Expression Omnibus (GEO) database and quantitative reverse transcription polymerase chain reaction (qRT-PCR). The biological function of EEF1A2 in OS was studied using cell counting kit-8 (CCK8) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay, Transwell assay, and OS of xenograft nude mice model. Real-time fluorescence quantitative PCR was used to detect the expression level of EEF1A2 mRNA in OS tissues and cell lines. Western blot was used to detect the phosphorylation level of Akt and mTOR.
There was high expression of EEF1A2 in OS, which was closely related to the Enneking stage and tumor size of OS. , EEF1A2 promoted the proliferation, migration, and invasion of OS cells; , EEF1A2 promoted the growth of OS tumors. The mechanism study showed that EEF1A2 can promote protein kinase B (Akt) and mammalian target of rapamycin (mTOR) phosphorylation, thereby activating the Akt/mTOR signaling pathway in OS.
There is high expression of EEF1A2 in OS, which can promote the proliferation, migration, and invasion of OS cells and the growth of OS tumors in vivo via activation of the Akt/mTOR signaling pathway.
骨肉瘤（OS）是儿童和青少年中常见的骨癌，会导致大量与癌症相关的死亡。真核翻译延伸因子1α2（EEF1A2）已被揭示具有致癌特性，并在许多癌症中促进肿瘤进展。我们想要研究EEF1A2在骨肉瘤中的生物学功能和机制。
使用基因表达综合数据库（GEO）和定量逆转录聚合酶链反应（qRT-PCR）研究EEF1A2在骨肉瘤中的表达。使用细胞计数试剂盒-8（CCK8）检测、5-乙炔基-2'-脱氧尿苷（EdU）检测、Transwell检测以及异种移植裸鼠模型的骨肉瘤研究EEF1A2在骨肉瘤中的生物学功能。实时荧光定量PCR用于检测骨肉瘤组织和细胞系中EEF1A2 mRNA的表达水平。蛋白质免疫印迹法用于检测Akt和mTOR的磷酸化水平。
骨肉瘤中EEF1A2表达较高，这与骨肉瘤的Enneking分期和肿瘤大小密切相关。EEF1A2促进骨肉瘤细胞的增殖、迁移和侵袭；EEF1A2促进骨肉瘤肿瘤的生长。机制研究表明，EEF1A2可促进蛋白激酶B（Akt）和雷帕霉素靶蛋白（mTOR）磷酸化，从而激活骨肉瘤中的Akt/mTOR信号通路。
骨肉瘤中EEF1A2表达较高，其可通过激活Akt/mTOR信号通路促进骨肉瘤细胞的增殖、迁移和侵袭以及体内骨肉瘤肿瘤的生长。
