Pál Sára E, Tóth Renáta, Nosanchuk Joshua D, Vágvölgyi Csaba, Németh Tibor, Gácser Attila
Department of Microbiology, University of Szeged, Közép Fasor, 6726 Szeged, Hungary.
Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY 10461, USA.
J Fungi (Basel). 2021 Jan 29;7(2):97. doi: 10.3390/jof7020097.
Relative to the vast data regarding the virulence mechanisms of , there is limited knowledge on the emerging opportunistic human pathogen The aim of this study was to generate and characterize an overexpression mutant collection to identify and explore virulence factors in . With the obtained mutants, we investigated stress tolerance, morphology switch, biofilm formation, phagocytosis, and in vivo virulence in larvae and mouse models. In order to evaluate the results, we compared the data from the overexpression collection analysis to the results derived from previous deletion mutant library characterizations. Of the 37 overexpression mutants, we identified eight with altered phenotypes compared to the controls. This work is the first report to identify , , , , and as contributors to virulence by regulating functions associated with host-pathogen interactions and biofilm formation. Our findings also confirmed the role of , and in pathogenesis. This study was the first attempt to use an overexpression strategy to systematically assess gene function in , and our results demonstrate that this approach is effective for such investigations.
相对于关于[病原体名称未给出]毒力机制的大量数据，对于新兴的机会性人类病原体[病原体名称未给出]的了解有限。本研究的目的是构建并表征一个过表达突变体文库，以鉴定和探索[病原体名称未给出]中的毒力因子。利用获得的突变体，我们在果蝇幼虫和小鼠模型中研究了其应激耐受性、形态转换、生物膜形成、吞噬作用和体内毒力。为了评估结果，我们将来自[病原体名称未给出]过表达文库分析的数据与先前缺失突变体文库表征的结果进行了比较。在37个[病原体名称未给出]过表达突变体中，我们鉴定出8个与对照相比具有改变表型的突变体。这项工作是首次报道通过调节与宿主 - 病原体相互作用和生物膜形成相关的功能，鉴定[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]和[基因名称未给出]是[病原体名称未给出]毒力的贡献因素。我们的发现还证实了[基因名称未给出]、[基因名称未给出]和[基因名称未给出]在发病机制中的作用。本研究是首次尝试使用过表达策略系统评估[病原体名称未给出]中的基因功能，我们的结果表明这种方法对于此类研究是有效的。
