用于研究丙型肝炎病毒的三维细胞培养系统。

Three-Dimensional Cell Culture Systems for Studying Hepatitis C Virus.

机构信息

Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA.

出版信息

Viruses. 2021 Jan 30;13(2):211. doi: 10.3390/v13020211.

DOI:10.3390/v13020211

PMID:33573191

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7911643/

Abstract

Hepatocytes, the major target of hepatitis C virus (HCV), are highly polarized. HCV infection requires extensive trafficking to distinct subcellular domains in the polarized hepatocyte. Polarized cells and three-dimensional organoids are commonly used to study liver functions and differentiation. Researchers have begun adapting these cell culture models that morphologically and physiologically resemble hepatocytes in vivo to study HCV infection. This review summarizes the use of three-dimensional cell culture systems in studies of HCV infection.

摘要

肝细胞是丙型肝炎病毒 (HCV) 的主要靶标，具有高度极化的特征。HCV 感染需要在极化的肝细胞中广泛运输到不同的亚细胞区域。极化细胞和三维类器官常用于研究肝脏功能和分化。研究人员已开始采用这些在形态和生理上类似于体内肝细胞的细胞培养模型来研究 HCV 感染。本综述总结了三维细胞培养系统在 HCV 感染研究中的应用。