Wilson N W, Macartney J C
Department of Histopathology, St Thomas's Hospital, London.
Lab Invest. 1988 Apr;58(4):459-65.
In order to determine whether organ culture of gastric mucosa preserves in vivo kinetic alterations, a comparison of in vivo and in vitro measurements of cell proliferation was made on antral mucosa from rats treated with the carcinogen methyl-nitro-nitrosoguanidine, administered orally for up to 30 weeks. Morphologic, morphometric and cell kinetic features in gastric mucosa were observed in vivo in treated and control rats. Similar in vitro measurements were made on mucosa from treated and control rats after a further 24-hour organ culture. All carcinogen-treated stomachs showed erosions, with widespread areas of glandular architectural irregularity which were more widespread after 30 weeks carcinogen treatment. Organ cultures showed good preservation of these morphologic features. In areas of architecturally normal mucosa, the carcinogen caused an increase in antral pit vertical column counts. This alteration was preserved after organ culture of carcinogen-treated mucosa. Cell birth rates and [3H]thymidine flash labeling indices were raised in architecturally normal mucosa from carcinogen-treated animals. These kinetic indices continued to be elevated after organ culture, though the in vitro values were lower than in vivo. Cell birth rates in architecturally abnormal mucosa from carcinogen-treated animals did not differ significantly from adjacent morphologically normal mucosa. Despite evidence of epithelial cell loss with depression of kinetic activity on organ culture compared with in vivo results, organ culture gives consistent results which reflect methyl-nitroso-nitrosoguanidine-induced changes and is of value for further in vitro investigations.
为了确定胃黏膜的器官培养是否能保留体内的动力学改变，对口服给予致癌剂甲基硝基亚硝基胍长达30周的大鼠胃窦黏膜进行了体内和体外细胞增殖测量的比较。观察了经处理的大鼠和对照大鼠胃黏膜的形态学、形态计量学和细胞动力学特征。在进一步进行24小时器官培养后，对经处理的大鼠和对照大鼠的黏膜进行了类似的体外测量。所有经致癌剂处理的胃均出现糜烂，腺管结构不规则的区域广泛，在致癌剂处理30周后更为广泛。器官培养显示这些形态学特征得到了很好的保留。在结构正常的黏膜区域，致癌剂导致胃窦小凹垂直柱计数增加。经致癌剂处理的黏膜进行器官培养后，这种改变得以保留。致癌剂处理动物的结构正常黏膜中的细胞出生率和[3H]胸腺嘧啶闪光标记指数升高。这些动力学指标在器官培养后仍持续升高，尽管体外值低于体内值。致癌剂处理动物的结构异常黏膜中的细胞出生率与相邻形态正常的黏膜无显著差异。尽管与体内结果相比，器官培养显示上皮细胞丢失且动力学活性降低，但器官培养给出了一致的结果，反映了甲基硝基亚硝基胍诱导的变化，对进一步的体外研究具有价值。
