State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China.
University of Science and Technology of China, Hefei, Anhui 230026, China.
Anal Chem. 2021 Feb 23;93(7):3461-3469. doi: 10.1021/acs.analchem.0c04783. Epub 2021 Feb 12.
Organic molecules and related nanomaterials have attracted extensive attention in the realm of electrochemiluminescence (ECL). Herein, a well-known electroluminescence (EL) dopant 2,3,6,7-tetrahydro-1,1,7,7,-tetramethyl-1,5,11-10-(2-benzothiazolyl)quinolizino-[9,9a,1gh] coumarin (C545T) is selected as a new ECL illuminant, which shows a high photoluminescence quantum yield of nearly 100% and excellent ECL performance in the organic phase. For utilizing C545T to achieve ECL detection in aqueous solution, organic microrods of C545T (C545T MRs) were synthesized by a precipitation method. Cyclic voltammetry and differential pulse voltammetry of C545T and C545T MRs in acetonitrile or phosphate buffer showed one reduction and multiple oxidation peaks, suggesting that the multiple charge states of C545T could be produced by continuous electron- or hole-injection processes. The annihilated ECL emission of C545T and C545T MRs was observed using ECL transient technology. In the presence of triethanolamine (TEOA) or potassium persulfate (KSO), C545T MRs can also give bright anodic and cathodic ECL emission at the GCE/water interface. The proposed ECL system not only has multichannel ECL emission but also shows intense yellow emission (569 nm) with a relative ECL efficiency of 0.81 when TEOA was used as a coreactant. Benefiting from the strong ECL emission of the C545T MRs/TEOA system and the quenching effect of dopamine (DA) on ECL, a convenient sensor for DA was developed with high selectivity and sensitivity.
有机分子和相关的纳米材料在电致化学发光（ECL）领域引起了广泛关注。在此，选择一种著名的电致发光（EL）掺杂剂 2,3,6,7-四氢-1,1,7,7-四甲基-1,5,11-10-（2-苯并噻唑基）喹啉[9,9a,1gh]香豆素（C545T）作为新的 ECL 发光体，其在有机相中具有近 100%的高光致发光量子产率和优异的 ECL 性能。为了利用 C545T 在水溶液中实现 ECL 检测，通过沉淀法合成了 C545T 的有机微棒（C545T MRs）。C545T 和 C545T MRs 在乙腈或磷酸盐缓冲液中的循环伏安法和差分脉冲伏安法显示出一个还原和多个氧化峰，表明 C545T 的多个电荷态可以通过连续的电子或空穴注入过程产生。使用 ECL 瞬态技术观察到 C545T 和 C545T MRs 的湮灭 ECL 发射。在三乙醇胺（TEOA）或过硫酸钾（KSO）存在下，C545T MRs 也可以在 GCE/水界面上产生明亮的阳极和阴极 ECL 发射。所提出的 ECL 系统不仅具有多通道 ECL 发射，而且当 TEOA 用作共反应物时，还显示出强烈的黄色发射（569nm），相对 ECL 效率为 0.81。受益于 C545T MRs/TEOA 系统的强 ECL 发射和多巴胺（DA）对 ECL 的猝灭效应，开发了一种用于 DA 的方便传感器，具有高选择性和灵敏度。
