Chen Jianxin, Lin Min, Che Yan, Guo Jian, Lin Wei
The First Department of Gastrointestinal Surgery, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China.
Gastrointestinal Surgery Research Institute, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China.
Oncol Lett. 2021 Mar;21(3):194. doi: 10.3892/ol.2021.12455. Epub 2021 Jan 8.
In recent years, among all patients with colorectal cancer, the proportion of young patients has been gradually increasing. However, the molecular mechanisms involved in colorectal cancer in the young are largely unknown. In the present study the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas datasets were integrated to elucidate the key gene biomarkers in these patients. The GSE41657 and GSE41258 datasets were downloaded from the GEO database. By screening for differentially expressed genes, Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein-protein interaction analysis, hub gene screening and survival analysis, two key genes, CXCL8 and VEGFA, which were enriched in cancer pathways, were obtained. Reverse transcription-quantitative (RT-q)PCR was performed to verify the outcome obtained by bioinformatics analysis. In conclusion, the present study identified two key genes using bioinformatics analysis and RT-qPCR validation. These results indicated that the candidate genes may be involved in the progression of colorectal cancer in young people, and these two genes may act as ideal prognostic indicators or therapeutic targets for colorectal cancer in the youth.
近年来，在所有结直肠癌患者中，年轻患者的比例一直在逐渐增加。然而，年轻人结直肠癌所涉及的分子机制在很大程度上尚不清楚。在本研究中，整合了基因表达综合数据库（GEO）和癌症基因组图谱数据集，以阐明这些患者中的关键基因生物标志物。从GEO数据库下载了GSE41657和GSE41258数据集。通过筛选差异表达基因、基因本体分析、京都基因与基因组百科全书通路分析、蛋白质-蛋白质相互作用分析、枢纽基因筛选和生存分析，获得了两个在癌症通路中富集的关键基因，即CXCL8和VEGFA。进行逆转录定量（RT-q）PCR以验证通过生物信息学分析获得的结果。总之，本研究通过生物信息学分析和RT-qPCR验证鉴定出两个关键基因。这些结果表明，候选基因可能参与年轻人结直肠癌的进展，并且这两个基因可能作为年轻人结直肠癌的理想预后指标或治疗靶点。
