Dai Yanan, Peng Li, Zhang Xiaohua, Wu Qingjing, Yao Jie, Xing Qiu, Zheng Yunyan, Huang Xiaobo, Chen Shaomei, Xie Qing
Department of Ophthalmology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan, P.R. China, Haikou, China.
Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, Changsha, China.
PeerJ. 2021 Jan 29;9:e10667. doi: 10.7717/peerj.10667. eCollection 2021.
This study aimed to evaluate the effects of coconut water on the general condition (fasting blood sugar and body weight) and retina of diabetic rats.
Forty-eight Sprague-Dawley male rats were divided into normal controls (NC), diabetes mellitus (DM), diabetes+coconut water (DM+CW), and diabetes+glibenclamide (DM+Gli) groups. After 4 weeks of normal feeding, coconut water was given to group III-DM+CW and 0.6 mg/kg glibenclamide to group IV-DM+Gli. The blood sugar, body weight, total retinal thickness, pathological changes, and VEGF expression in the retina were analyzed at different time points.
The fasting blood sugar was 4-6 mmol/L in group I-NC and continuously increased in group II-DM, whereas gradually decreased after the 4th experiment week in the remaining two groups. The rats, except in group I-NC, have lost weight. In group II-DM, the total retinal thickness was significantly increased after the 8th and 12th experiment week, and the pathological changes in retina were observed. VEGF was almost fully expressed in the ganglion cell layer and inner granular layer and partially expressed in the outer granular layer in group II-DM, and mainly expressed in the ganglion cell layer and inner layer in group I-NC, with a lighter color. Group III-DM + CW and group IV-DM + Gli demonstrated similar VEGF expression as in group I-NC.
Coconut water has the potential to reduce blood sugar and diabetic retinal damage, serving as a candidate drug or nutrient for treating diabetes and its complications.
本研究旨在评估椰汁对糖尿病大鼠一般状况（空腹血糖和体重）及视网膜的影响。
将48只雄性Sprague-Dawley大鼠分为正常对照组（NC）、糖尿病组（DM）、糖尿病+椰汁组（DM+CW）和糖尿病+格列本脲组（DM+Gli）。正常喂养4周后，给III组-DM+CW给予椰汁，给IV组-DM+Gli给予0.6mg/kg格列本脲。在不同时间点分析血糖、体重、视网膜总厚度、病理变化及视网膜中VEGF表达。
I组-NC空腹血糖为4-6mmol/L，II组-DM持续升高，而其余两组在实验第4周后逐渐下降。除I组-NC外，大鼠体重均减轻。II组-DM在实验第8周和第12周后视网膜总厚度显著增加，并观察到视网膜病理变化。II组-DM中VEGF在神经节细胞层和内颗粒层几乎完全表达，在外颗粒层部分表达，I组-NC主要在神经节细胞层和内层表达，颜色较浅。III组-DM+CW和IV组-DM+Gli的VEGF表达与I组-NC相似显示。
椰汁具有降低血糖和糖尿病视网膜损伤的潜力，可作为治疗糖尿病及其并发症的候选药物或营养物质。
