Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, CP 44430, Guadalajara, Jalisco, México.
CONACYT, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, CP 44430, Guadalajara, Jalisco, México.
Bioprocess Biosyst Eng. 2021 Jun;44(6):1155-1166. doi: 10.1007/s00449-021-02512-y. Epub 2021 Feb 11.
The selection of a suitable growth regime can increase the physiological performance of microalgae and improve bioprocess based on these microorganisms from agro-industrial residues. Thus, this study assessed the biotechnology capacity-biomass production, biochemical composition, and nutrient uptake-from tequila vinasses (TVs) as the nutrient source of three indigenous microalgae-Chlorella sp., Scenedesmus sp., and Chlamydomonas sp.-cultured under heterotrophic and mixotrophic conditions. The results demonstrated that under the mixotrophic regime, the three microalgae evaluated reached the highest nitrogen uptake, biomass production, and cell compound accumulation. Under this condition, Chlorella sp. and Scenedesmus sp. showed the highest nutrient uptake and biomass production, 1.7 ± 0.3 and 1.9 ± 0.3 g L, respectively; however, the biochemical composition, mainly carbohydrates and proteins, varied depending on the microalgal strain and its growth regime. Overall, our results demonstrated the biotechnological capacity of native microalgae from TVs, which may vary not only depending on the microalgal strain but also the culture strategy implemented and the characteristics of the residue used, highlighting-from a perspective of circular bio-economy-the feasibility of implementing microalgal bioprocess to reuse and valorize the nutrimental composition of TVs through biomass and high-valuable metabolite production, depicting a sustainable strategy for tequila agro-industry in Mexico.
选择合适的生长方式可以提高微藻的生理性能，并基于农业工业废料中的这些微生物改进生物工艺。因此，本研究评估了龙舌兰酒酒糟（TVs）作为三种本土微藻（绿球藻、栅藻和小球藻）的营养源的生物技术能力——生物量生产、生化成分和养分吸收——在异养和混合营养条件下。结果表明，在混合营养条件下，所评估的三种微藻达到了最高的氮吸收、生物量生产和细胞化合物积累。在这种条件下，绿球藻和栅藻表现出最高的养分吸收和生物量生产，分别为 1.7±0.3 和 1.9±0.3 g/L；然而，生化成分，主要是碳水化合物和蛋白质，取决于微藻菌株及其生长方式而有所不同。总体而言，我们的结果表明了来自 TVs 的本土微藻的生物技术能力，这不仅取决于微藻菌株，还取决于所实施的培养策略和所使用的残渣的特性，从循环生物经济的角度突出了实施微藻生物工艺的可行性，通过生物量和高价值代谢产物的生产来回收和增值 TVs 的营养成分，为墨西哥的龙舌兰酒农业产业描绘了一种可持续的策略。
