Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology (CPMB & B), Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India.
Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology (CPMB & B), Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India.
Curr Microbiol. 2021 Oct;78(10):3770-3781. doi: 10.1007/s00284-021-02643-1. Epub 2021 Sep 6.
Enhanced biofuel production strategies from microalgae by employing affordable bio-waste usage are fetching significance, nowadays. This study examines the effect of VWE for enhanced biomass from new indigenous microalgal isolates, Asterarcys sp. SPC, Scenedesmus sp. KT-U, Scenedesmus sp. KTWL-A, Coelastrum sp. T-E, and Chlorella sp. TWL-B. The growth of microalgae in VWE-treated growth media showed considerable increase (1.14-2.3 folds) than control medium (without VWE). Further, two effective native microalgae were selected based on growth in VWE treatment, biomass productivity, and TAG accumulation through statistical clustering analysis. Mixotrophic batch cultivation of Scenedesmus sp. KT-U and Asterarcys sp. SPC cultivated using VWE treatment in the optimum concentration had produced significant average increase in BP (1.8 and 1.4 folds, respectively) than control (without VWE). Whereas in the lipid production phase, there was a noticeable increase in lipid yield in VWE-treated cells of lipid phase (231.8 ± 17.9 mg/L and 243.5 ± 25 mg/L) in Scenedesmus sp. KT-U and Asterarcys sp. SPC, respectively, than in control (140.5 ± 28 mg/L and 166.4 ± 23 mg/L) with considerable TAG accumulation. Thus, this study imparts strain selection process of native microalgae based on vegetable waste usage for improved yield of biomass and lipid amenable for cost-effective biodiesel production.
采用经济实惠的生物废料利用来增强微藻生物燃料的生产策略具有重要意义。本研究考察了 VWE 对新本土微藻分离株 Asterarcys sp. SPC、Scenedesmus sp. KT-U、Scenedesmus sp. KTWL-A、Coelastrum sp. T-E 和 Chlorella sp. TWL-B 增强生物量的影响。在 VWE 处理的生长培养基中，微藻的生长显示出相当大的增加（1.14-2.3 倍）比对照培养基（无 VWE）。此外，根据 VWE 处理中的生长、生物量生产力和 TAG 积累，通过统计聚类分析选择了两种有效的本土微藻。在最优浓度下，利用 VWE 处理进行混养批培养的 Scenedesmus sp. KT-U 和 Asterarcys sp. SPC 产生了显著的平均 BP 增加（分别为 1.8 和 1.4 倍）比对照（无 VWE）。而在脂质生产阶段，VWE 处理的脂质相中 Scenedesmus sp. KT-U 和 Asterarcys sp. SPC 的细胞脂质产量有明显增加（分别为 231.8±17.9mg/L 和 243.5±25mg/L）比对照（无 VWE）（140.5±28mg/L 和 166.4±23mg/L），同时 TAG 积累量也相当可观。因此，本研究基于蔬菜废物利用对本土微藻进行了菌株选择过程，以提高生物量和脂质的产量，有利于经济高效的生物柴油生产。
