基于数字微流控技术的 DNA 甲基化的灵敏、快速和自动化检测。

Sensitive, Rapid, and Automated Detection of DNA Methylation Based on Digital Microfluidics.

机构信息

The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Xiamen 361005, China.

出版信息

ACS Appl Mater Interfaces. 2021 Feb 24;13(7):8042-8048. doi: 10.1021/acsami.0c21995. Epub 2021 Feb 12.

DOI:10.1021/acsami.0c21995

PMID:33576594

Abstract

Biomarkers based on DNA methylation have attracted wide attention in biomedical research due to their potential clinical value. Therefore, a sensitive and accurate method for DNA methylation detection is highly desirable for the discovery and diagnostics of human diseases, especially cancers. Here, an integrated, low-cost, and portable point-of-care (POC) device is presented to analyze DNA methylation, which integrates the process of pyrosequencing in a digital microfluidic chip. Without additional equipment and complicated operation, droplets are manipulated by patterned electrodes with individually programmed control. The system exhibited an excellent sensitivity with a limit of detection (LOD) of 10 pg and a comparable checkout down to 5% methylation level within 30 min, which offered a potential substitute for the detection of DNA methylation. With the advantages of portability, ease of use, high accuracy, and low cost, the POC platform shows great potential for the analysis of tumor-specific circulating DNA.

摘要

基于 DNA 甲基化的生物标志物因其潜在的临床价值而在生物医学研究中受到广泛关注。因此，一种灵敏、准确的 DNA 甲基化检测方法对于人类疾病，特别是癌症的发现和诊断具有重要意义。本文提出了一种集成的、低成本的、便携的即时检测（POC）设备，用于分析 DNA 甲基化，该设备将焦磷酸测序过程集成在数字微流控芯片中。该设备无需额外的设备和复杂的操作，通过具有单独编程控制的图案化电极来操控液滴。该系统表现出优异的灵敏度，检测限（LOD）为 10 pg，在 30 分钟内可检测到低至 5%甲基化水平的甲基化，为 DNA 甲基化检测提供了一种潜在的替代方法。POC 平台具有便携、易用、高精度和低成本的优势，在分析肿瘤特异性循环 DNA 方面具有很大的潜力。