Key Laboratory of Spectrochemical Analysis and Instrumentation, Ministry of Education, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Pen-Tung Sah Institute of Micro-Nano Science and Technology, Discipline Intelligent Instrument & Equipment, Xiamen University, Xiamen 361005, China.
Zhonghe Gene Technology Co., Ltd., Tianjin 300308, China.
Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2410164121. doi: 10.1073/pnas.2410164121. Epub 2024 Aug 15.
In the age of information explosion, the exponential growth of digital data far exceeds the capacity of current mainstream storage media. DNA is emerging as a promising alternative due to its higher storage density, longer retention time, and lower power consumption. To date, commercially mature DNA synthesis and sequencing technologies allow for writing and reading of information on DNA with customization and convenience at the research level. However, under the disconnected and nonspecialized mode, DNA data storage encounters practical challenges, including susceptibility to errors, long storage latency, resource-intensive requirements, and elevated information security risks. Herein, we introduce a platform named DNA-DISK that seamlessly streamlined DNA synthesis, storage, and sequencing on digital microfluidics coupled with a tabletop device for automated end-to-end information storage. The single-nucleotide enzymatic DNA synthesis with biocapping strategy is utilized, offering an ecofriendly and cost-effective approach for data writing. A DNA encapsulation using thermo-responsive agarose is developed for on-chip solidification, not only eliminating data clutter but also preventing DNA degradation. Pyrosequencing is employed for in situ and accurate data reading. As a proof of concept, DNA-DISK successfully stored and retrieved a musical sheet file (228 bits) with lower write-to-read latency (4.4 min of latency per bit) as well as superior automation compared to other platforms, demonstrating its potential to evolve into a DNA Hard Disk Drive in the future.
在信息爆炸的时代，数字数据的指数级增长远远超过了当前主流存储介质的容量。由于 DNA 具有更高的存储密度、更长的保持时间和更低的功耗，因此它作为一种有前途的替代品而出现。迄今为止，商业化成熟的 DNA 合成和测序技术允许在研究层面上以定制化和便利的方式在 DNA 上写入和读取信息。然而，在不连续和非专业化的模式下，DNA 数据存储会遇到实际挑战，包括易出错、存储延迟长、资源密集型要求和信息安全风险增加。在这里，我们引入了一个名为 DNA-DISK 的平台，该平台在数字微流控技术上无缝地集成了 DNA 合成、存储和测序，以及一个用于自动化端到端信息存储的台式设备。该平台采用具有生物封端策略的单核苷酸酶促 DNA 合成，为数据写入提供了一种环保且经济高效的方法。开发了一种使用热响应性琼脂糖的 DNA 封装方法，用于芯片上的固化，不仅消除了数据混乱，而且还防止了 DNA 降解。焦磷酸测序用于原位和准确的数据读取。作为概念验证，DNA-DISK 成功地存储和检索了一张乐谱文件（228 位），其写入-读取延迟（每位 4.4 分钟的延迟）更低，与其他平台相比具有更高的自动化程度，表明其有潜力在未来演变成一种 DNA 硬盘驱动器。
