School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705, United States.
Department of Experimental Medical Science, Lund University, Lund 221 00, Sweden.
Org Lett. 2021 Mar 5;23(5):1738-1741. doi: 10.1021/acs.orglett.1c00168. Epub 2021 Feb 12.
Proteoglycans have important biological activities. To improve the overall synthetic efficiency, a new chemoenzymatic route has been established for the proteoglycan linkage region bearing a galactose-xylose disaccharide. The xylosylated glycopeptides were synthesized via solid phase synthesis, which was followed by the addition of the galactose unit by the galactosyl transferase β4GalT7. This work leads to a better understanding of the acceptor preference of β4GalT7 and opens the door for expeditious synthesis of the proteoglycan linkage region.
蛋白聚糖具有重要的生物学活性。为了提高整体合成效率,建立了一种新的化学酶法途径,用于连接带有半乳糖-木糖二糖的蛋白聚糖连接区。通过固相合成合成了糖基化肽,然后通过β4GalT7 半乳糖基转移酶添加半乳糖单元。这项工作有助于更好地了解β4GalT7 的受体偏好性,并为快速合成蛋白聚糖连接区打开了大门。