Lin Po-Han, Huang Xuefei
Department of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, 48824, USA.
Chembiochem. 2025 May 27;26(10):e202500095. doi: 10.1002/cbic.202500095. Epub 2025 May 16.
Proteoglycans (PGs) play roles in many important biological events, including growth factor signaling, wound repair, blood coagulation, brain development, and neural stem cell migration. As the glycosaminoglycan chain of PGs is attached to the core protein through a tetrasaccharide linkage region, it is important to decipher the intricate control of the multitude of enzymes engaged in biosynthesis of the linkage region. During the past two decades, significant advances have been achieved in our understanding of the identity and interactions of these enzymes as well as the control of their activities. Furthermore, the knowledge gained on biosynthesis of the linkage region has enabled the synthesis of structurally well-defined proteoglycan-associated glycopeptides. The expression, activity, and substrate profile of enzymes and the latest development of chemoenzymatic synthesis of linkage region-bearing glycopeptides are summarized in this review.
蛋白聚糖（PGs）在许多重要的生物学事件中发挥作用，包括生长因子信号传导、伤口修复、血液凝固、大脑发育和神经干细胞迁移。由于PGs的糖胺聚糖链通过四糖连接区域连接到核心蛋白上，因此解读参与连接区域生物合成的众多酶的复杂调控机制非常重要。在过去的二十年中，我们对这些酶的身份、相互作用以及它们活性的控制方面取得了重大进展。此外，在连接区域生物合成方面获得的知识使得能够合成结构明确的蛋白聚糖相关糖肽。本文综述了这些酶的表达、活性和底物谱以及含连接区域糖肽的化学酶促合成的最新进展。
