College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
Hospital of Stomatology, Jilin University, Changchun, 130021, China.
Cell Tissue Res. 2021 May;384(2):313-324. doi: 10.1007/s00441-020-03355-x. Epub 2021 Feb 12.
Previous studies have shown the effects of vitamins on the development of the mammary gland. However, the role of niacin in this process has not been reported. Therefore, the aim of this study is to investigate the effects of niacin on mammary gland development in pubertal mice and to use a mouse mammary epithelial cell line to study the underlying mechanism. The results showed that niacin could activate the AKT/mTOR and ERK signaling pathways and increase phosphorylation of 4EBP1 to promote the synthesis of cell proliferation markers, leading to the dissociation of the Rb-E2F1 complex in mMECs. In addition, 0.5% niacin promoted mammary duct development, increased the expression of cyclin D1/D3 and PCNA and activated Akt/mTOR and ERK1/2 in the mammary glands of pubertal mice. These results strongly suggest that niacin stimulates mammary gland development in pubertal mice through the Akt/mTOR and ERK1/2 signaling pathways.
先前的研究已经表明了维生素对乳腺发育的影响。然而，烟酸在这个过程中的作用尚未被报道。因此，本研究旨在探讨烟酸对青春期小鼠乳腺发育的影响，并利用小鼠乳腺上皮细胞系来研究其潜在的机制。结果表明，烟酸可以激活 AKT/mTOR 和 ERK 信号通路，并增加 4EBP1 的磷酸化，从而促进细胞增殖标志物的合成，导致 Rb-E2F1 复合物在 mMECs 中的解离。此外，0.5%的烟酸促进乳腺导管发育，增加了 cyclin D1/D3 和 PCNA 的表达，并在青春期小鼠的乳腺中激活了 Akt/mTOR 和 ERK1/2。这些结果强烈表明，烟酸通过 Akt/mTOR 和 ERK1/2 信号通路刺激青春期小鼠的乳腺发育。
