BHF Center for Cardiovascular Science, Queen's Medical Research Institute, Edinburgh, UK.
Centre for Regenerative Medicine, Edinburgh, UK.
Methods Mol Biol. 2021;2235:13-26. doi: 10.1007/978-1-0716-1056-5_2.
The physiological, pathological, and regenerative roles of pericytes as microvascular mural cells and multipotent precursors have gained significant attention. The capacity to prospectively purify pericytes from multiple organs enables the investigation of their tissue-specific regenerative capabilities. Here, we describe the application of purified human pericytes for cardiac regeneration post-infarct in an immunodeficient mouse model. This protocol includes experimental details of pericyte isolation from both human skeletal and cardiac muscle, an immunodeficient mouse model of acute myocardial infarction, and xenogeneic pericyte transplantation.
周细胞作为微血管壁细胞和多能前体细胞的生理、病理和再生作用引起了人们的极大关注。能够从多个器官中前瞻性地纯化周细胞，使人们能够研究它们的组织特异性再生能力。在这里，我们描述了从人类骨骼肌和心肌中分离纯化的周细胞在免疫缺陷小鼠急性心肌梗死模型中的心脏再生应用。本方案包括从人类骨骼肌和心肌中分离周细胞、免疫缺陷型急性心肌梗死小鼠模型以及异种周细胞移植的实验细节。
