Aix Marseille Univ, INSERM, MMG, Marseille, France.
Methods Mol Biol. 2021;2235:61-87. doi: 10.1007/978-1-0716-1056-5_6.
The goal of lineage tracing is to understand body formation over time by discovering which cells are the progeny of a specific, identified, ancestral progenitor. Subsidiary questions include unequivocal identification of what they have become, how many descendants develop, whether they live or die, and where they are located in the tissue or body at the end of the window examined. A classical approach in experimental embryology, lineage tracing continues to be used in developmental biology and stem cell and cancer research, wherever cellular potential and behavior need to be studied in multiple dimensions, of which one is time. Each technical approach has its advantages and drawbacks. This chapter, with some previously unpublished data, will concentrate nonexclusively on the use of interspecies chimeras to explore the origins of perivascular (or mural) cells, of which those adjacent to the vascular endothelium are termed pericytes for this purpose. These studies laid the groundwork for our understanding that pericytes derive from progenitor mesenchymal pools of multiple origins in the vertebrate embryo, some of which persist into adulthood. The results obtained through xenografting, like in the methodology described here, complement those obtained through genetic lineage-tracing techniques within a given species.
谱系追踪的目的是通过发现特定的、已识别的祖细胞的后代，来了解身体随时间的形成过程。辅助性问题包括明确识别它们变成了什么、有多少后代发育、它们是存活还是死亡，以及在检查结束时它们位于组织或体内的何处。这是实验胚胎学中的一种经典方法，谱系追踪在发育生物学、干细胞和癌症研究中仍在使用，只要需要在多个维度（其中一个是时间）研究细胞的潜力和行为。每种技术方法都有其优点和缺点。本章将集中讨论使用种间嵌合体来探索血管周围（或壁）细胞的起源，其中与血管内皮相邻的细胞为此目的被称为周细胞，同时也包含一些之前未发表的数据。这些研究为我们理解周细胞来源于脊椎动物胚胎中多个来源的祖细胞间质池奠定了基础，其中一些在成年后仍然存在。通过异种移植获得的结果，如这里描述的方法，可以补充在给定物种内通过遗传谱系追踪技术获得的结果。
