人多能干细胞衍生的周细胞。
Derivation of Pericytes from Human Pluripotent Stem Cells.
机构信息
Orthopaedic Hospital Research Center, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
出版信息
Methods Mol Biol. 2021;2235:119-125. doi: 10.1007/978-1-0716-1056-5_8.
Human pluripotent stem cells (hPSCs), either embryonic or induced, offer a plentiful platform for derivation of multiple cell types. Pericytes, generated from hPSCs, are multipotent precursors with vasculogenic features that exhibit high proliferation capability in long-term cultures. Administration of hPSC-pericytes into ischemic murine hind limb is associated with therapeutic angiogenesis and attenuation of muscle wasting. Here, we describe the protocol for derivation of large numbers of pericytes from spontaneously differentiating hPSC-embryoid bodies.
人多能干细胞（hPSCs），无论是胚胎来源的还是诱导多能干细胞来源的，都为衍生多种细胞类型提供了丰富的平台。从 hPSCs 中产生的周细胞是具有血管生成特征的多能前体细胞，在长期培养中具有高增殖能力。将 hPSC-周细胞注射到缺血性小鼠后肢中与治疗性血管生成和肌肉减少症的衰减有关。在这里，我们描述了从自发分化的 hPSC 类胚体中大量衍生周细胞的方案。
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