使用核酸模拟物-荧光原位杂交技术对多菌株或多物种生物膜系统中单个细菌的相互作用和空间排列进行表征。
Characterization of Social Interactions and Spatial Arrangement of Individual Bacteria in MultiStrain or Multispecies Biofilm Systems Using Nucleic Acid Mimics-Fluorescence In Situ Hybridization.
机构信息
LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
出版信息
Methods Mol Biol. 2021;2246:97-109. doi: 10.1007/978-1-0716-1115-9_7.
Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm , and when coupled with confocal scanning laser microscopy (CSLM), it enables the visualization of the three-dimensional (3D) structure of the biofilm and the spatial arrangement of each individual species/strain within it. In this chapter, we describe the protocol for characterizing multistrain or multispecies biofilm formation using NAM-FISH and CSLM.
生物膜通常由不同的细菌和真菌物种/菌株组成，它们通过彼此之间的社会相互作用形成复杂的结构。荧光原位杂交（FISH）可以帮助我们识别生物膜中存在的不同物种/菌株，当与共聚焦扫描激光显微镜（CSLM）结合使用时，它可以使生物膜的三维（3D）结构和其中每个单独物种/菌株的空间排列可视化。在本章中，我们描述了使用 NAM-FISH 和 CSLM 表征多菌株或多物种生物膜形成的方案。
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