LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Methods Mol Biol. 2021;2246:97-109. doi: 10.1007/978-1-0716-1115-9_7.
Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm , and when coupled with confocal scanning laser microscopy (CSLM), it enables the visualization of the three-dimensional (3D) structure of the biofilm and the spatial arrangement of each individual species/strain within it. In this chapter, we describe the protocol for characterizing multistrain or multispecies biofilm formation using NAM-FISH and CSLM.
生物膜通常由不同的细菌和真菌物种/菌株组成，它们通过彼此之间的社会相互作用形成复杂的结构。荧光原位杂交（FISH）可以帮助我们识别生物膜中存在的不同物种/菌株，当与共聚焦扫描激光显微镜（CSLM）结合使用时，它可以使生物膜的三维（3D）结构和其中每个单独物种/菌株的空间排列可视化。在本章中，我们描述了使用 NAM-FISH 和 CSLM 表征多菌株或多物种生物膜形成的方案。
