Chang Xiaowen, Dong Shan, Bai Wenliang, Di Yan, Gu Ruijuan, Liu Fuguo, Zhao Beita, Wang Yutang, Liu Xuebo
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Shenzhen Academy of Metrology and Quality Inspection, National Nutrition Food Testing Center (Guangdong), Shenzhen 518000, China.
J Agric Food Chem. 2021 Feb 24;69(7):2179-2189. doi: 10.1021/acs.jafc.0c07521. Epub 2021 Feb 12.
Chicoric acid (CA) can display health benefits as a dietary polyphenol. However, as CA is widely metabolized , the actual compounds responsible for its bioactivities are not entirely known. Herein, the major methylated metabolites of CA were isolated from an co-incubation system, and their structures were elucidated. The antioxidant activities of the monomethylated metabolites (M1) and dimethylated metabolites (M2) of CA were evaluated against HO-induced oxidative stress damage in HepG2 cells and compared to CA. The results indicated that both M1 and M2 had better antioxidant capacities than CA by increasing cell viability, improving mitochondrial function, and balancing cellular redox status. These compounds also prevented oxidative stress by mediating the Keap1/Nrf2 transcriptional pathway and downregulating enzyme activity. The current research indicates that the methylated metabolites of CA could potentially be the candidates that are responsible for the biological efficacies attributed to CA.
菊苣酸（CA）作为一种膳食多酚，具有有益健康的特性。然而，由于CA广泛代谢，其生物活性的实际负责化合物并不完全清楚。在此，从共孵育系统中分离出CA的主要甲基化代谢产物，并阐明了它们的结构。评估了CA的单甲基化代谢产物（M1）和二甲基化代谢产物（M2）对HepG2细胞中HO诱导的氧化应激损伤的抗氧化活性，并与CA进行了比较。结果表明，M1和M2通过提高细胞活力、改善线粒体功能和平衡细胞氧化还原状态，比CA具有更好的抗氧化能力。这些化合物还通过介导Keap1/Nrf2转录途径和下调酶活性来预防氧化应激。目前的研究表明，CA的甲基化代谢产物可能是赋予CA生物学功效的候选物质。
