16750University of Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.
The Royal Veterinary College, 3163University of London, Hertfordshire, UK.
Vet Pathol. 2021 May;58(3):503-507. doi: 10.1177/0300985820985126. Epub 2021 Feb 12.
This study describes the clinical and pathological characteristics of cutaneous spindle cell squamous cell carcinoma (SCSCC) in 18 cats. The average age of the cats was 11.8 ± 2.7 years, and all tumors were located in the facial skin, mainly affecting the pinna (13/18, 72%), followed by the periorbital area (4/18, 22%) and the dorsal muzzle (1/18, 6%). Tumors were composed of fusiform neoplastic cells with moderate atypia arranged in solid sheets or fascicles with foci of squamous differentiation. A panel of antibodies against cytokeratins, vimentin, S-100 protein, NSE, GFAP, Melan A, SMA, desmin, CD18, CD31, and p63 was used to help differentiate SCSCC from other spindle cell malignancies. SCSCCs expressed CK5/6 (17/18, 94%), AE1/AE3 (15/18, 83%), and p63 protein (18/18, 100%), but there was no immunolabeling for CK8/18. A role for sunlight exposure in the pathogenesis of the tumors was suggested by changes indicative of actinic keratosis, the location of the tumors in dorsal areas, and the absence of histomorphologic features of papillomavirus infection. Recurrence was not recorded in 14/18 cases (78%) during a follow-up period of 7 to 25 months. Three of 18 (17%) tumors recurred or led to humane euthanasia due to local progression, and one case (5%) had regional lymph node metastasis. Clinical outcome varied with cutaneous location, mitotic count, and invasion of surgical margins; thus, SCSCCs with a more aggressive behavior were located in the periorbital area (4/4 cases), had ≥14 mitoses in 10 high-power fields (2.37 mm) (4/4 cases), and showed invasion of surgical margins (3/4 cases).
本研究描述了 18 只猫的皮肤梭形细胞鳞状细胞癌 (SCSCC) 的临床和病理特征。猫的平均年龄为 11.8 ± 2.7 岁，所有肿瘤均位于面部皮肤，主要影响耳 (13/18，72%)，其次是眶周区域 (4/18，22%) 和鼻背 (1/18，6%)。肿瘤由中度异型的梭形肿瘤细胞组成，呈实性片或束状排列，伴有鳞状分化灶。一组针对细胞角蛋白、波形蛋白、S-100 蛋白、NSE、GFAP、Melan A、SMA、结蛋白、CD18、CD31 和 p63 的抗体用于帮助区分 SCSCC 与其他梭形细胞恶性肿瘤。SCSCC 表达 CK5/6(17/18，94%)、AE1/AE3(15/18，83%)和 p63 蛋白(18/18，100%)，但 CK8/18 无免疫标记。肿瘤位于背部区域以及无乳头瘤病毒感染的组织形态学特征，提示日光暴露在肿瘤发病机制中起作用。在 18 例中有 14 例 (78%)在 7 至 25 个月的随访期间未记录复发。由于局部进展，18 例中有 3 例 (17%)肿瘤复发或导致安乐死，1 例 (5%)有区域淋巴结转移。临床结果与皮肤位置、有丝分裂计数和手术切缘侵犯有关；因此，具有更具侵袭性行为的 SCSCC 位于眶周区域 (4/4 例)，在 10 个高倍视野中具有≥14 个有丝分裂 (2.37mm)(4/4 例)，并显示手术切缘侵犯 (3/4 例)。
