Rapp G, Güth K
Abteilung Biophysik, Max-Planck-Institut für medizinische Forschung, Heidelberg, Federal Republic of Germany.
Pflugers Arch. 1988 Feb;411(2):200-3. doi: 10.1007/BF00582315.
Novel techniques of flash photolysis experiments require high intensity light sources in the near UV. We describe here a simple and inexpensive flash device which may complete with bulky and expensive laser systems if the experiments do not necessitate very short light pulses. Using a particular optical arrangement and stored electrical energy, a variation of the parameters voltage and capacitance led to a difference in light output by a factor of more than two. The system is used to relax both skeletal and smooth muscle fibres in the rigor state by releasing up to 2 mM ATP from 12.5 mM caged-ATP.
闪光光解实验的新技术需要近紫外光区域的高强度光源。在此,我们描述一种简单且廉价的闪光装置,如果实验不需要极短的光脉冲,那么该装置可与庞大且昂贵的激光系统相媲美。利用一种特殊的光学装置和储存的电能,改变电压和电容参数会使光输出产生两倍多的差异。该系统通过从12.5 mM笼形ATP中释放高达2 mM的ATP,用于使处于僵直状态的骨骼肌纤维和平滑肌纤维松弛。