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A method for the manufacture of single barrel liquid ion-selective microelectrodes: an in situ study of ant venom pH.

作者信息

Howl J D, von Sicard N A, Publicover S J, Anderson M

机构信息

Department of Zoology and Comparative Physiology, University of Birmingham, Great Britain.

出版信息

Pflugers Arch. 1988 Feb;411(2):212-5. doi: 10.1007/BF00582317.

DOI:10.1007/BF00582317
PMID:3357759
Abstract

A method for the manufacture of single barrel ion-sensitive microelectrodes, employing liquid ion-selective sensors, is described in detail. This method had proven reliable and repeatable for the manufacture of pH-sensitive microelectrodes using a liquid proton carrier. It is believed that this method has advantages over other published methods since it allows direct visualization and control of major steps during electrode fabrication and overcomes several problems often encountered using more conventional methods. Microelectrodes fabricated using this technique have been used to measure the in situ pH of venom from two myrmicine ants, Tetramorium caespitum (L.) and Myrmica ruginodis (N.).

摘要

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本文引用的文献

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Science. 1959 Aug 21;130(3373):449. doi: 10.1126/science.130.3373.449.
2
Neutral carrier ion-selective microelectrodes for measurement of intracellular free calcium.用于测量细胞内游离钙的中性载体离子选择性微电极。
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Neutral carrier based hydrogen ion selective microelectrode for extra- and intracellular studies.用于细胞外和细胞内研究的基于中性载体的氢离子选择性微电极。
Bay K 8644在体外诱导小鼠骨骼肌坏死:肌纤维分解先于细胞内[Ca]或pH的显著变化。
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Microelectrode determination of oxyntic cell pH in intact frog gastric mucosa. Effect of histamine.
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Intracellular activity of potassium in normal and dystrophic skeletal muscle from C57BL/6J mice.C57BL/6J小鼠正常和营养不良性骨骼肌中钾的细胞内活性
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Intracellular calcium and sodium activity in sheep heart Purkinje fibres. Effect of changes of external sodium and intracellular pH.绵羊心脏浦肯野纤维中的细胞内钙和钠活性。细胞外钠和细胞内pH变化的影响。
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6
Free calcium in sheep cardiac tissue and frog skeletal muscle measured with Ca2+-selective microelectrodes.用钙离子选择性微电极测量绵羊心脏组织和青蛙骨骼肌中的游离钙。
Pflugers Arch. 1984 Sep;402(1):1-9. doi: 10.1007/BF00584824.
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Hydrogen ion buffers for biological research.用于生物学研究的氢离子缓冲剂。
Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011.
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Rapid changes of potassium concentration at the outer surface of exposed single neurons during membrane current flow.膜电流流动期间,暴露的单个神经元外表面钾离子浓度的快速变化。
J Gen Physiol. 1973 Mar;61(3):385-99. doi: 10.1085/jgp.61.3.385.
9
Submicron tip breakage and silanization control improve ion-selective microelectrodes.亚微米级尖端破损和硅烷化控制改善离子选择性微电极。
Am J Physiol. 1985 Nov;249(5 Pt 1):C514-21. doi: 10.1152/ajpcell.1985.249.5.C514.
10
Measurement of intracellular calcium ion activity with neutral exchanger ion sensitive microelectrodes.使用中性交换剂离子敏感微电极测量细胞内钙离子活性
Cell Calcium. 1985 Apr;6(1-2):57-68. doi: 10.1016/0143-4160(85)90034-x.