• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Non-radioactive automated sequencing of oligonucleotides by chemical degradation.通过化学降解对寡核苷酸进行非放射性自动测序。
Nucleic Acids Res. 1988 Mar 25;16(5):2203-6. doi: 10.1093/nar/16.5.2203.
2
Automated sequencing of fluorescently labelled DNA by chemical degradation.通过化学降解对荧光标记的DNA进行自动测序。
DNA Seq. 1990;1(1):63-71. doi: 10.3109/10425179009041348.
3
Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.使用带有多种荧光团的寡脱氧核苷酸引物进行连续在线DNA测序。
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5610-4. doi: 10.1073/pnas.85.15.5610.
4
The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.5' 端含脂肪族氨基的寡核苷酸的合成：用于DNA序列分析的荧光DNA引物的合成。
Nucleic Acids Res. 1985 Apr 11;13(7):2399-412. doi: 10.1093/nar/13.7.2399.
5
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides.一种使用荧光链终止双脱氧核苷酸进行快速DNA测序的系统。
Science. 1987 Oct 16;238(4825):336-41. doi: 10.1126/science.2443975.
6
Non-radioactive chemical sequencing of biotin labelled DNA.生物素标记DNA的非放射性化学测序
Nucleic Acids Res. 1989 Mar 25;17(6):2181-6. doi: 10.1093/nar/17.6.2181.
7
DNA sequencing by the dideoxy method.双脱氧法DNA测序
Curr Protoc Mol Biol. 2001 May;Chapter 7:Unit7.4A. doi: 10.1002/0471142727.mb0704as47.
8
Fluorescence chemistries for automated primer-directed DNA sequencing.用于自动引物定向DNA测序的荧光化学方法。
Electrophoresis. 1992 Aug;13(8):552-9. doi: 10.1002/elps.11501301113.
9
Fluorescent dye phosphoramidite labelling of oligonucleotides.寡核苷酸的荧光染料亚磷酰胺标记
Nucleic Acids Symp Ser. 1992(27):99-100. doi: 10.1002/chin.199302246.
10
Ultrasensitive near-IR fluorescence detection for capillary gel electrophoresis and DNA sequencing applications.用于毛细管凝胶电泳和DNA测序应用的超灵敏近红外荧光检测
Anal Chem. 1995 Oct 1;67(19):3427-32. doi: 10.1021/ac00115a010.

引用本文的文献

1
An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins.一种砷荧光化合物作为一种新型探针来研究砷结合蛋白。
Protein J. 2012 Dec;31(8):656-66. doi: 10.1007/s10930-012-9441-6.
2
Chemical methods of DNA and RNA fluorescent labeling.DNA和RNA荧光标记的化学方法。
Nucleic Acids Res. 1996 Nov 15;24(22):4535-42. doi: 10.1093/nar/24.22.4535.
3
Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin.单分子荧光检测：自相关标准及藻红蛋白的实验实现
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4087-91. doi: 10.1073/pnas.86.11.4087.
4
Non-radioactive chemical sequencing of biotin labelled DNA.生物素标记DNA的非放射性化学测序
Nucleic Acids Res. 1989 Mar 25;17(6):2181-6. doi: 10.1093/nar/17.6.2181.
5
Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA.使用DNA体外扩增进行直接基因组荧光在线测序与分析。
Nucleic Acids Res. 1989 Apr 11;17(7):2517-27. doi: 10.1093/nar/17.7.2517.

本文引用的文献

1
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.聚合物载体寡核苷酸合成 XVIII：脱氧核苷的β-氰基乙基-N，N-二烷基氨基-/N-吗啉代亚磷酰胺用于 DNA 片段合成，简化最终产物的脱保护和分离
Nucleic Acids Res. 1984 Jun 11;12(11):4539-57. doi: 10.1093/nar/12.11.4539.
2
Solid-phase methods for sequencing of nucleic acids I. Simultaneous sequencing of different oligodeoxyribonucleotides using a new, mechanically stable anion-exchange paper.核酸测序的固相方法I. 使用一种新型机械稳定阴离子交换纸同时对不同寡脱氧核糖核苷酸进行测序
Nucleic Acids Res. 1985 Feb 25;13(4):1173-84. doi: 10.1093/nar/13.4.1173.
3
Fluorescence detection in automated DNA sequence analysis.自动DNA序列分析中的荧光检测。
Nature. 1986;321(6071):674-9. doi: 10.1038/321674a0.
4
A simple and rapid preparation of M13 sequencing templates for manual and automated dideoxy sequencing.一种用于手动和自动双脱氧测序的M13测序模板的简单快速制备方法。
Nucleic Acids Res. 1987 Jul 24;15(14):5507-16. doi: 10.1093/nar/15.14.5507.
5
Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis.自动化DNA测序：电泳过程中荧光条带的超灵敏检测。
Nucleic Acids Res. 1987 Jun 11;15(11):4593-602. doi: 10.1093/nar/15.11.4593.
6
A non-radioactive automated method for DNA sequence determination.一种用于DNA序列测定的非放射性自动化方法。
J Biochem Biophys Methods. 1986 Dec;13(6):315-23. doi: 10.1016/0165-022x(86)90038-2.
7
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides.一种使用荧光链终止双脱氧核苷酸进行快速DNA测序的系统。
Science. 1987 Oct 16;238(4825):336-41. doi: 10.1126/science.2443975.

通过化学降解对寡核苷酸进行非放射性自动测序。

Non-radioactive automated sequencing of oligonucleotides by chemical degradation.

作者信息

Ansorge W, Rosenthal A, Sproat B, Schwager C, Stegemann J, Voss H

机构信息

European Molecular Biology Laboratory, Heidelberg, FRG.

出版信息

Nucleic Acids Res. 1988 Mar 25;16(5):2203-6. doi: 10.1093/nar/16.5.2203.

DOI:10.1093/nar/16.5.2203
PMID:3357772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338209/
Abstract

A non-radioactive sequencing of fluorescently labelled oligonucleotides by solid-phase chemical degradation is described. Although non-radioactive methods have been reported for the dideoxy chain termination technique, such a method has not yet been developed for the chemical degradation sequencing of DNA fragments. A 21-mer fluorescein labelled M13 sequencing primer was sequenced in an on-line automated system in about 30 minutes. The fluorescent dye and its bond to the oligonucleotide were stable during the chemical reactions used for the base specific degradations. As the sequence is determined on-line during electrophoresis, reloading and running 10 fragments simultaneously allows us to use one gel for sequencing of about 50 different oligonucleotides.

摘要

本文描述了通过固相化学降解对荧光标记寡核苷酸进行非放射性测序的方法。虽然已经报道了用于双脱氧链终止技术的非放射性方法，但尚未开发出用于DNA片段化学降解测序的此类方法。一个21聚体荧光素标记的M13测序引物在在线自动化系统中约30分钟内完成了测序。在用于碱基特异性降解的化学反应过程中，荧光染料及其与寡核苷酸的连接是稳定的。由于序列在电泳过程中在线确定，同时重新加载并运行10个片段使我们能够用一块凝胶对约50种不同的寡核苷酸进行测序。