Hawkins T L, Du Z, Halloran N D, Wilson R K
MRC Laboratory of Molecular Biology, Cambridge.
Electrophoresis. 1992 Aug;13(8):552-9. doi: 10.1002/elps.11501301113.
A major limitation in the applicability of automated DNA sequencing instruments has been the difficulty in using user-defined oligonucleotide primers which allow sequencing reactions to start at any specific point in a region of interest. Recently, new chemistries have become available for fluorescent labeling which will begin to facilitate the use of any oligonucleotide primer with automated DNA sequencers. In this report, we describe several methods for automated primer-directed DNA sequencing, and compare and discuss the relative merits and limitations of these methods.
自动化DNA测序仪应用中的一个主要限制是使用用户定义的寡核苷酸引物存在困难，这些引物可使测序反应在感兴趣区域的任何特定点起始。最近，出现了用于荧光标记的新化学方法，这将开始便于在自动化DNA测序仪中使用任何寡核苷酸引物。在本报告中，我们描述了几种自动化引物导向DNA测序的方法，并比较和讨论了这些方法的相对优缺点。
